Princeton University

Harvard College

Thomas J Silhavy

Daniel E Kahne

Dorothee Andres

Matthew Lebar

Marcin Grabowicz

Howard A Shuman

Jon Beckwith

Maxime Schwartz

The present disclosure generally relates to genetic engineering of bacteria. More particularly, the present disclosure describes genetic engineering ofto create mutant O-antigen ligase, as well as novel lipopolysaccharide molecules resulting from that genetic engineering. Methods for using those novel molecules are also described.

Modified lipopolysaccharide glycoform and method of use

This invention is a method for fusing, within a bacterial host, a gene for a cytoplasmic protein to a gene for a non-cytoplasmic protein, so that the hybrid protein produced is transported to, near, or beyond the cell surface for ease of collection and purification, or for immunological use. The invention also encompasses the fused gene created by the disclosed method.

Fused gene and method of making and using same

Growing community of inventors

Belle Mead, NJ, United States of America

Thomas J Silhavy