Other

Radiometer Corporate Development Ltd.

Thomas J Sands

Robert Andrew Badley

Anthony Hugh Carr

Ian Jobling

Robert A Badley

Anthony H Carr

A test for microbial growth is carried out by culturing a microorgnism to be tested in a lliquid culture medium containing an enzymatically hydrolyzable fluorogenic substrate for a period of less than about 7 hours, measuring fluorescence of the culture medium resulting from hydrolysis of the fluorogenic substrate by growth of the microorganism, continuing culturing of the microorganism in the culture medium for an additional period, and measuring microorganism growth by a non-fluorescent method as a confirmatory test of growth determined from fluorescence. Preferably, the additional culture period is overnight and the non-fluorescent method is by visual inspection. The culture medium may be in the wall of a microtitre plate, and the culture medium can contain an antibiotic to test for sensitivity of a microorganism to the antibiotic or to test for minimum inhibitory concentration of the antibiotic.

Microorganism testing with a hydrolyzable fluorogenic substrate

A method of determining the susceptibility of microorganisms to an antimicrobial substance wherein the microorganisms are cultured in the presence of the antimicrobial substance, and growth or lack of growth is assessed by detecting a fluorescent product resulting from the enzymatic hydrolysis of a fluorogenic substrate. The fluorogenic substrates include 7-N-(aminoacyl)-7-amido-4-methylcoumarin, 7-N-(alanyl)-7-amido-4-methylcoumarin, or a combination of 7-N-(alanyl)-7-amido-4-methylcoumarin and 4-methyl umbelliferyl phosphate.

Microbial susceptibility assay using

Processes and materials for carrying out microchemical and

Growing community of inventors

Wellingborough, United Kingdom

Thomas J Sands