United States of America As Represented by the Secretary of the Air Force

University of Cincinnati

Thomas J Lamkin

Daniel J Hassett

Chloe J A Shea

Deborah E Taylor

Roland Saldanha

Warunya Panmanee

Shengchang Su

Umesh Adiga

Daniel Cowan

Patrick McLendon

Robert Trevino

A high content analysis computing system, computer program product and method provides receiving two or more sets of microscopic images of respective sites of selected biological cell assay. Each set of microscopic images comprises one or more images having an aggregate field of view sufficient to encompass all of the cells within the respective site and a resolution sufficient to image cellular bodies within each of encompassed cells. For each set of microscopic images, a library of algorithms is executed that evaluate image features for each cell in each site producing a quantified feature measurement for each cell. Quantified feature measurements are compared for each one of the two or more sets of microscopic images. A subset of the library of algorithms is identified that discriminate at least two of the two sets or more microscopic images and deployed as a computer program product for evaluating additional biological cell assays.

Unbiased feature selection in high content analysis of biological image samples

Stable, constitutively expressed, chromosomal fluorescent transcriptional fusions in bacterial pathogens and methods of using the same to screen candidate compounds for anti-bacterial efficacy.

Modified bacterial pathogens and methods for effectuating rapid response to contamination by known or unknown bacterial pathogens

A high through-put screening method for identifying agents effective for inhibiting biofilm formation and/or killing established biofilm are disclosed. The method includes three tiers, and each tier includes three specific biological process assays. The tier levels are a primary screen, a confirmation screen, and a dose-response screen, and the biological process assays include assays for total bacterial growth, bacterial metabolic activity, and biofilm formation. The series of assays may be run once or more than once at each tier. A library of compounds is subject to tier A and only compounds meeting a primary parameter advance to tier B, and only tier B compounds meeting a confirmation parameter advance to tier C, and only tier C compounds meeting a dose-response parameter are identified as putative agents effective for inhibiting and/or eradicating a biofilm, further wherein the assays are conducted for each compound subject to the respective tier. The method is effective and validated for identifying agents which inhibit and/or kill, andbiofilms. Agents identified according to the high through-put screen and validated in follow-up experiments as effective for inhibiting and/or killing bacterial biofilms are also disclosed.

Multi-tiered high through-put screen for compounds effective against bacterial biofilm and compounds effective for inhibiting and eradicating bacterial biofilm

A high through-put screening method for identifying agents effective for inhibiting biofilm formation and/or killing established biofilm are disclosed. The method includes three tiers, and each tier includes three specific biological process assays. The tier levels are a primary screen, a confirmation screen, and a dose-response screen, and the biological process assays include as says for total bacterial growth, bacterial metabolic activity, and biofilm formation.

Multi-tiered, high through-put screen for compounds effective against bacterial biofilm compounds effective for inhibiting and eradicating bacterial biofilm

The present invention includes live strain ofwherein a gene selected from the group consisting of priA and purA is inactivated. The present invention further includes a pharmaceutical composition comprising one or more live strains ofaccording to the present invention and a pharmaceutically acceptable carrier. The present invention further includes a method of using one or more live strains ofaccording to the present invention to confer immunity against a virulent strain of. The method comprises administering an effective amount of one or more live strains ofaccording to the present invention or a pharmaceutical composition comprising one or more livestrains to an animal such that an immune response is produced in the animal.

[object Object]

A method of classifying, with a computer processor, at least one feature of cells from a low contrast, digital image. The method includes generating a contrast-enhanced image by applying a high-pass filter to the low contrast, digital image. The contrast-enhanced image is smoothed with a first low pass filter. A background image, generated from the low contrast, digital image, is subtracted from the smoothed, contrast-enhanced image to form an analysis image. The at least one feature is identified in analysis image.

Growing community of inventors

Cincinnati, OH, United States of America

Thomas J Lamkin