The United States of America As Represented by the Secretary of

United States of America, As Represented by the Secretary of Agriculture

Thomas J Jacks

Thomas P Hensarling

Kanniah Rajasekaran

Thomas E Cleveland

Jeffrey W Cary

Lawrence Y Yatsu

Karl-Heinz Van Pee

Linda L Muller

Robert H Barker

The present invention provides a method of making plants resistant to plant pathogens by transformation of host cells with a nucleic acid encoding a nonheme haloperoxidase. Transgenic plants which express a nonheme chloroperoxidase thereby resulting in enhanced resistance to phytopathogens are provided.

Transformation of plants with a chloroperoxidase gene to enhance disease resistance

A novel process for increasing the solubility of dry oilseed protein is dlosed. Proteins, such as, peanut, pumpkin, and soybean are mixed with 30% hydrogen peroxide at about 1:20 ratio. The mixture is centrifuged at approximately 2000 g for about 15 minutes to clarify the solution. The supernatant is dialyzed against water containing catalase and the dialysate is freeze-dried.

Solubilization of dry protein in aqueous or acidic media after treatment

A solvent system composed of ethanol-acetonitrile-water dissolved certain membrane proteins of biological origin. The solvent system was optically transparent to ultraviolet radiation from 200 to 400 nanometers (nm). This transparency permitted ascertainment of spectral characteristics of membrane proteins at these wavelengths.

Solubilization of protein with ethanol-acetonitril-water solvent system

Hexane and mixtures of hexane containing 2 to 25% acetic acid (v/v) were used to prepare oil and protein from glanded cottonseed by solvent extraction. As the amount of acetic acid in the solvent increased, the amounts of total lipid, phospholipid, neutral oil, and gossypol in each miscella increased, but the amount of free fatty acids did not change significantly. The solubility of the protein in 0.02N NaOH did not decrease until the amount of acetic acid in the solvent used to prepare each meal increased to a point between 4 and 10 percent. The drainage of the miscella (solvent-lipid solution) through the marc (insoluble residue) was much more rapid when the solvent was hexane-acetic acid than when it was hexane alone.

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Metairie, LA, United States of America

Thomas J Jacks