Degussa Aktiengesellschaft

Evonik Degussa Gmbh

Other

Degussa Gmbh

Evonik Operations Gmbh

Thomas Hermann

Walter Pfefferle

Mike Farwick

Brigitte Bathe

Achim Marx

Mechthild Rieping

Caroline Kreutzer

Bettina Moeckel

Georg Thierbach

Michael Binder

Stephan Hans

Bettina Möckel

Christine Bastuck

Klaus Huthmacher

Bettina M&ouml;ckel

Jörn Kalinowski

Bettina Mockel

Natalie Schischka

Alfred Pühler

Andreas Karau

Günter Knaup

Alfred P&uuml;hler

Friedhelm Merz

Indra Schroeder

Ulrich Becker

J&ouml;rn Kalinowski

IIona Walger

Klaus Erich Uffmann

Michael Buchholz

Stefan Hans

Birgit Witteck

Monika Martens

Indra Schr&ouml;der

Mike Garwick

The present invention relates to a fermentation process for the preparation of L-amino acids in which the following steps are carried out: (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms' open reading frames of yjfA and ytfP are individually or jointly inactivated by one or more methods of mutagenesis selected from the group consisting of deletion, insertional mutagenesis due to homologous recombination, and transition or tranversion mutagenesis with incorporation of a non-sense mutation in the yjfA and ytfP gene region; (b) concentration of the fermentation broth to eliminate water and increase the concentration of said L-amino acids; and (c) isolation of the L-amino acids.

[object Object]

A process for the production of L-amino acids, in particular L-threonine, in which the following steps are carried out:

Process for the production of L-amino acids using strains of the family enterobacteriaceae that contain an attenuated fruR gene

Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

Nucleotide sequences for encoding of the lysR2-gene

The invention relates to sulfur-containing animal-feed additives produced from fermentation liquors, and to processes for their production.

Sulfur-containing animal-feed additives

The invention relates to a process for the preparation of L-amino acids, especially L-lysine, L-valine, L-homoserine and L-threonine, by fermenting a microorganism of the genuswhich has a mutation or deletion in the gene encoding aspartate ammonium lyase (aspA). This mutation results in the loss of aspA enzymatic activity in the microorganism

Enterobacteriaceae strains with an attenuated aspA gene for the fermentative production of amino acids

An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising:

Process for preparing L-amino acids with corynebacteria with enhanced nucleotide sequences coding for pknD

The invention related to an isolated nucleic acid consisting of a fragment of the polynucleotide ofcitA gene or a fragment of the full complement of the polynucleotide of thecitA gene wherein said fragment is at least 30 consecutive nucleotide in length, and the vector and host cell harboring these nucleic acids.

A process for the production of an L-amino acid wherein coryneform bacteria (e.g.) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.

Process for the production of L-amino acids by fermentation using coryneform bacteria

The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.

Nucleotide sequences which code for the eno gene

Nucleotide sequences coding for the pknB gene

The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.

Nucleotide sequences which code for the ccpA2 gene

Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of

The invention relates to a process for producing an L-amino acid, in particular, L-threonine, which comprises: cultivating a microorganism of the family Enterobacteriaceae producing the desired L-amino acid under conditions suitable for production of the amino acid, in which the fruR (cra) (fructose repressor) gene or nucleotide secluences coding therefor in the microorganism is attenuated, in particular are switched off or eliminated, and recovering the L-amino acid from the medium or in the cells of the microorganism.

Process for the production of L-amino acids using strains of the family Enterobacteriaceae that contain an attenuated fruR gene

Process for the production of L-amino acids by fermentation using coryneform bacteria with an attenuated mqo gene

The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the sigC gene from, and a host-vector system having a coryneform host bacterium, such as, and a vector which carries at least the sigC gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Methods for producing amino acids in coryneform bacteria using an enhanced sigC gene

Growing community of inventors

Bielefeld, Germany

Thomas Hermann