C-lecta Gmbh

Roche Diagnostics Operations, Inc.

Thomas Greiner-Stoeffele

Marc Struhalla

Stefan Schoenert

Andreas Vogel

Claudia Feller

Rico Czaja

Daniel Schwarze

Sabrina Koepke

Ramona Schmiedel

Hedda Merkens

Kamila Rzeznicka

Andreas Petri

Meike Hoffmann

The present disclosure provides the sequence of apreproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

Methods of recombinantly producing neutral protease originating from

Expression vector encoding aneutral protease

The invention relates to ketoreductases and the use thereof. The ketoreductases of the invention are particularly useful for enzymatically catalyzing the reduction of ketones to chiral secondary alcohols.

Ketoreductases

The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

Recombinantly produced neutral protease originating from Paenibacillus polymyxa

An expression vector including two separately inducible converging promoters P1 and P2, and expression system including such an expression vector and an additional regulator vector, a method of protein expression using such an expression system, and a method of investigating (meta)genome libraries using such an expression system.

Expression vector

A method for generating a variant library of DNA sequences starting from at least one DNA starting sequence and including the steps: a) selecting at least two mutation sites in the starting sequence; b) dividing the DNA starting sequence into at least two sequence segments such that at least two of these sequence segments each contain at least one of the mutation sites; c) amplifying the sequence segments by polymerase chain reaction using at least five different oligonucleotides, where at (i) least one of the oligonucleotides can attach to each mutation site; (ii) at least two of the oligonucleotides can attach to at least one mutation site, and (iii) mutations are introduced, via mismatch positions, into the PCR amplificates by the oligonucleotides at the mutation sites where at least two mutations are introduced at at least one of the mutation sites; and d) linking the amplificates to give DNA sequences.

Process for generating a variant library of DNA sequences

A method for producing a nuclease of a gram negative bacterium or a nuclease preparation containing a nuclease of a gram negative bacterium including expression of the nuclease in a gram positive bacterium and subsequent secretion of the nuclease, as well as a nuclease or a nuclease preparation that can be obtained by this method.

Method for producing serratia marcescens nuclease using a bacillus expression host

The invention relates to the use of an amidohydrolase for preparing foodstuffs or stimulants.

Heat-stable asparaginase for reducing acrylamide in food stuffs or stimulants

The invention relates to a nitrilase having improved activity in the reaction of a nitrile to form the corresponding carboxylic acid, in particular with respect to reacting 2-methylglutaronitrile, 1-(cyanomethyl)cyclohexane-1-carbonitrile, and benzonitrile. The nitrilase according to the invention is related to nitrilase from

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Leipzig, Germany

Thomas Greiner-Stoeffele