Millipore Corporation

Thomas G Zermani

Donald B Rising

James C Groves

Aldo M Pitt

Joseph John Raneri

Sara Gutierrez

Jeffrey Busnach

Carolyn Lee

Phillip Clark

Brian D Foley

Kenneth G Desilets

The present invention provides a mechanical lock for securing one or more filters within a filtration device and methods for producing the mechanical lock. The use of an interference fit punch causes a portion of the inner surface of the wall to be skived and rolled along the wall until it reaches the desired location where it forms a mechanical crimp to retain the one or more filters within the device. In one embodiment, the inner surface has an inwardly taper. In the other, it has straight walls or outwardly tapered walls. The use of a gasket such as an O-ring on top of the filter before the wall is skived provides a liquid tight seal.

Method for making a mechanical interlock for filters

A fluid filtering device includes a plurality of filtering wells and an angling mechanism which adjusts the angle of the membranes within the filtering wells relative to a line of a centrifuge where the line is perpendicular to the axis of rotation of a centrifuge and passes through the center of a major plane of the filtering device. The angling mechanism can include a wedge located between the center of rotation of a centrifuge and a swinging bucket of the centrifuge. The angling mechanism can also be located within each filtering well and can provide individually specified angles for each membrane, relative to the line of the centrifuge.

Methods for filtering fluids

Fluid filtering device

A single or multiwell plate using membrane, preferably an ultrafiltration membrane to filter selected solutes from a liquid is disclosed. In particular, a preferred construction and method of sealing the membrane to the interior of the well so as to form an integral seal between the top portion of the well and the bottom portion is disclosed. The preferred method is to use a heat seal device which secures the filter to the underdrain portion of the device.

Method of forming well(s) containing filtration devices

Well(s) containing filtration devices

A single or multiwell plate using membrane, preferably an ultrafiltration membrane to filter selected solutes from a liquid is disclosed. In particular, a preferred construction and method of sealing the membrane to the interior of the well so as to form an integral seal between the top portion of the well and the boftom portion is disclosed. The preferred method is to use a heat seal device which secures the filter to the underdrain portion of the device.

A dialysis apparatus is provided including a multiwell plate, a separations membrane and a template having holes which mate with the wells. The membrane is sealed to one end of the wells by positioning the template about the outer peripheral surfaces of the wells with the membrane being frictionally held therebetween.

Multiwell test apparatus

A filter punch construction is provided for use in conjunction with a multiwell filtration device wherein each well includes a filtration membrane. The punch has a leading surface to which is secured an elongated piercing member having a sharp point and a periphery comprising holding or cutting edges and a following surface which has a guide member. The piercing member and cutting edges pierce the filtration membrane in each well and push the entire filter and retentate thereon into a receptacle vial.

Filter punch and filter collection system

A multi-well filtration apparatus for the assay of microliter quantities is provided wherein a filter is positioned in each well on a plate having an open spout positioned in the plate. The spout has a collar on its outer surface extending in a direction perpendicular to the vertical axis of the spout. The collar prevents a liquid droplet from climbing the outer surface of the spout from its open end.

Growing community of inventors

Peabody, MA, United States of America

Thomas G Zermani