University of California

Thomas G Graeber

Antoni Ribas

Hsian-Rong Tseng

Jennifer Tsoi

Yanju Wang

Nicolaos Palaskas

Cong Fang

Nam T Vu

Arion F Hadjioannou (Chatziioannou)

Lidia Robert

The current methods and compositions provide for a novel therapeutic method for treating patients diagnosed with melanoma, especially those that have become resistant to certain other therapies. Accordingly, certain aspects of the disclosure relate to a method for treating melanoma in a subject, the method comprising administering a composition comprising a ferroptosis-inducing agent or other dedifferentiated melanoma-targeting agent to the subject.

Methods and compositions for treating melanoma

An immunocapture-based in vitro kinase assay on an integrated polydimethylsiloxane (PDMS) microfluidics platform that can reproducibly measure kinase activity from as few as 3,000 cells is described. For this platform, the standard radiometricP-ATP labeled phosphate transfer assay was adopted. Implementation on a microfluidic device required the development of methods for repeated trapping and mixing of solid-phase affinity micro beads. A solid state beta-particle camera imbedded directly below the microfluidic device was used to provide real-time quantitative detection of the signal from this and other microfluidic radio bioassays. The integrated device can measure ABL protein kinase activity from BCR-ABL positive leukemia patient samples, and can measure the small molecule phosphorylation such as phosphorylation of the deoxycytidine analogF-FAC by deoxycytidine kinase (dCK) isolated from cell lysates.

Integrated microfluidic radioassay and imaging platform for small sample analysis

Growing community of inventors

Los Angeles, CA, United States of America

Thomas G Graeber