Research Corporation Technologies, Inc.

Biogrammatics, Inc.

Thomas G Chappell

Kurt R Gehlsen

James M Cregg

Knut R Madden

Ilya I Tolstorukov

Disclosed herein are novelstrains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., α-1,6-mannosyltransferase, or 'OCH1 protein'). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.

Pichia pastoris strains for producing predominantly homogeneous glycan structure

In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated fromwherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using thepromoters to produce proteins and polypeptides, and to the proteins and polypeptides produced using the expression methods.

Yeast promotors for protein expression

strains for producing predominantly homogeneous glycan structure

Isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are disclosed. More specifically, nucleic acids isolated frompastons having promoter activity and expression methods, host cells, expression vectors, and DNA constructs of using thepastons promoters to produce different proteins and polypeptides are disclosed.

Disclosed herein are novelstrains for expression of exogenous proteins with substantially homogeneous N-glycans. The strains are genetically engineered to include a mutant OCH1 allele which is transcribed into an mRNA coding for a mutant OCH1 gene product (i.e., &#x3b1;-1,6-mannosyltransferase, or 'OCH1 protein'). The mutant OCH1 protein contains a catalytic domain substantially identical to that of the wild type OCH1 protein, but lacks an N-terminal sequence necessary to target the OCH1 protein to the Golgi apparatus. The strains disclosed herein are robust, stable, and transformable, and the mutant OCH1 allele and the ability to produce substantially homogeneous N-glycans are maintained for generations after rounds of freezing and thawing and after subsequent transformations.

Yeast promoters for protein expression

This disclosure relates to noveldisplay systems, e.g., display systems featuring thestrains (such as SuperMan5) with substantially homogeneous N-glycans displayed on cell surface proteins.

Growing community of inventors

San Marcos, CA, United States of America

Thomas G Chappell