Basf Aktiengesellschaft

Basf Se Corporation

Thomas Friedrich

Bernhard Hauer

Rainer Stürmer

Michael Breuer

Siegfried Bialojan

Maria Keβeler

Thomas Subkowski

Reinhard Braatz

Elke Menkel-Conen

Christoph Kuenast

Hartwig Schröder

Maximilian Angel

Roland Kurth

Claus Bollschweiler

Ralf Mattes

Wolfgang Ladner

Volker Braig

Hansjoerg Rettenmaier

Christoph Nübling

Maria Kesseler

Martin Schmidt

Dietmar Haering

Hans-Joachim Hähnle

Burkhard Kroeger

Lidcay Herrera Taboada

Kurt Faber

Karl-Hermann Strube

Thomas Zelinski

Rainer St&uuml;rmer

Alexander Göthlich

Nina Schneider

Alexander Goethlich

Norbert Zimmermann

Wolfgang Koerwer

Jurgen Schweden

Lysander Chrisstoffels

Melanie Hall

Bettina Rosche

Rainer Stuermer

Martin Raditsch

Wolf-Rüdiger Krahnert

Andreas Schädler

Dirk Engels

Marion Ress-Löschke

Martin Rübenacker

Bernhard Haur

Andre Mueller

Sabine Rinck

Susanne Sabrowski

Dietmar Häring

Hartwig SCHRÖDER

Sabine Rinck-Jahnke

Process for producing optically active 3-aminocarboxylic acid ester compounds of general Formula I, and the ammonium salts thereof, in which in which Rand Rhave the meanings given above and Rstands for hydrogen, alkyl, cycloalkyl or aryl, is submitted to an enantioselective deacylation by adding a polypeptide according to claim

Amidase and use thereof for producing 3-aminocarboxylic acid esters

The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing optically active substituted alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.

Dehydrogenases, the derivatives thereof, and method for the production of optically active alkanols

A method for enzymatic preparation of compounds of the general formula (2) from unsaturated alkene derivatives of the general formula (1) by reducing a compound of the formula (1) in the presence of a reductase, comprising at least one of the polypeptide sequences SEQ ID NO: 1, 2 or 3 or having a functionally equivalent polypeptide sequence which is at least 80% identical to SEQ ID NO: 1, 2 or 3.

Process for enzymatic reduction of alkene derivatives

Provided is a process for esterifying or transesterifying side chains in polymers, which involves esterifying or transesterifying the carboxyl group having the Rradical of a copolymer obtained by polymerizing a (meth)acrylate of formula (I), and a monomer of formula (II): wherein Rand Rare independently H or CH, Rand Rare independently H or an alkyl radical which is optionally substituted by functional groups such as acryloyl, ether, amino, epoxy, halogen or sulfonic acid groups, and n is from 1 to 40, with an alcohol in the presence of an enzyme which catalyzes the esterifying or transesterifying. Further provided is the esterified or transesterified polymer product and its use.

Process for esterifying or transesterifying side chains in polymers

A process for preparing optically active saturated aldehydes or alcohols of the formula (2) from &#x3b1;,&#x3b2;-unsaturated aldehydes of the formula (1) by reduction in the presence of an enoate reductase

Process for the enzymatic preparation of citronellal

The invention relates to a process for the production of optically active Citronellal by enzymatic reduction of Citral with a reductase from 

Process for the production of citronellal

The invention relates to novel proteins from, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

Butynol I esterase

The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-typecomprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

Modified nitrilases and their use in methods for the production of carboxylic acids

A process for preparing optically active alkanols of the formula I  

Enzymatic reduction for producing optically active alcohols

The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and Rrepresents halogen, SH, OH, NO, NRRor NRRRX, wherein R, Rand Rindependently represent H or a lower alkyl or lower alkoxy group and Xrepresents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and Rare defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced. 

Method for producing optically active alcohols from alkanones using a dehydrogenase of azoarcus

The invention relates to novel proteins from, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

The present invention relates to enzymic and nonenzymic processes for preparing 3-methylamino-1-(thien-2-yl)propan-1-ol; as well as to enzymes for implementing these processes; and to nucleic acid sequences encoding these enzymes, to expression cassettes containing these nucleic acid sequences, to vectors and to recombinant hosts.

Methods for the production of 3-methylamino-1-(thiene-2-yl)-propane-1-ol

The invention relates to methods for reacting (di)amines as substrates in the presence of a lysine oxidase arid a reducing agent, resulting in alcohols, diols or cyclic secondary amines. In a particular embodiment, the invention is directed to methods of preparing cyclic secondary amines suitable for ultimately synthesizing piperidine-2-carboxylic acid and proline derivatives, useful, for example as thrombin inhibitors.

Reaction of (di)amines in the presence of a lysine oxidase and of a reducing agent

A polypeptide with pyruvate decarboxylase activity, derived from the pyruvate decarboxylase fromby substitution of an amino acid in position 553.

Production and use of pyruvate decarboxylase

The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

L-pantolactone-hydrolase and a method for producing D-pantolactone

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Darmstadt, Germany

Thomas Friedrich