Cytiva Sweden Ab

Ge Healthcare Bio-sciences Ab

Thomas Falkman

Patric Fricking

Henrik K Eriksson

Andreas Andersson

Christian Elnegard

Klaus Gebauer

Ralph Stankowski

Andreas Castan

Anders Wilen

Lars Johan Carlsson

Michael Miller

Camilla Estmer-Nilsson

Linnea Pauler

Claes Peterson

Eric Faldt

Tomas Yngve Agren

Rolf Hjorth

Patrick Jonsson

Johan L Carlsson

Shujian Yi

David Ronnholm

Nagaraj Raghavendra Rao

L Johan Carlsson

Claes Petterson

Linnea Troeng

Sahebagouda Alagur

Bineesh Kandoth

Christian ELNEGÅRD

Linnéa Pauler

The present subject matter discloses a bioreactor apparatus. The bioreactor apparatus comprises a bioreactor vessel configured to culture cells. The bioreactor apparatus furthermore comprises a sensor configured to measure Dissolved Oxygen (DO) in the bioreactor vessel. The DO measurements comprise a plurality of DO values recorded at, at least, a plurality of time instances during operation of the bioreactor apparatus. The bioreactor apparatus furthermore comprises a controller configured to obtain the DO measurements. The controller furthermore is to determine, in real-time or approximately real-time, an oxygen mass transfer co-efficient (kLa) associated with the operation of the bioreactor apparatus. Furthermore, the controller is configured to control, in real-time or approximately real-time, at least one cell culture parameter associated with the operation of the bioreactor apparatus based on the kLa.

Bioreactor apparatus and operation method thereof

The present invention relates to a bioreactor including at least one reactor vessel in form of a plastic bag, a tray () for holding said at least one bag, a rocking device for limited rocking motions of the tray () around a rocking axis (). According to the invention the bioreactor comprises a device () for enabling swinging of the tray () around a second axis () parallel to and distanced from the rocking axis ().

Bioreactor including a rocking device

The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.

Bag assembly for cultivation of cells

The invention discloses a condenser for a bioreactor exhaust, comprising: an inlet () adapted to be fluidically connected to a bioreactor exhaust port (), a cooling chamber () fluidically connected to the inlet and via a filter device () to an outlet (), a cooling conduit () in contact with the cooling chamber, a heating conduit () in contact with the filter device and a vortex tube () arranged to convey a cold gas stream through the cooling conduit and to convey a hot gas stream through the heating conduit.

Bioreactor condenser

A container comprising a first material and a second material, the first material being a flexible material and the second material being a rigid or semi-rigid material and at least partially defining an interior compartment for keeping a fluid inside the container characterized in that the flexible material and/or the semi-rigid or rigid material have thermoplastic properties and wherein the flexible material and the semi-rigid or rigid material are joined together by a seal or joint which comprises a heat seal and an adhesive seal.

Container with a seal and a method of making the seal

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Uppsala, Sweden

Thomas Falkman