University of Florida Research Foundation Inc

Georgia Tech Research Corporation

Fishing Physics, LLC

Marquez, Samantha M.

Thomas Ettor Angelini

Wallace Gregory Sawyer

Tapomoy Bhattacharjee

Steven Craig Ghivizzani

Brent S Sumerlin

Alberto Fernandez-Nieves

Ya-Wen Chang

Samantha M Marquez

C Parker Gibbs

Scott S Perry

Alexander Rudy

Kyle D Schulze

Carl David Crane

Kyle Gene Rowe

Curtis Taylor

Glyn Daniel Palmer

Christopher S O'Bryan

Joshua Muse

Meghan Hughes

Angela Athena Pitenis

Gregory Wallace Sawyer

Christopher P Kabb

Juan Manuel Uruena

Ryan M Nixon

William R Niederhauser

Wallace C Sawyer

Mike Norton

Gregory John Toupalik

Cameron Morley

Angela A Pitenis

A three-dimensional cell growth medium is described. The cell growth medium may comprise hydrogel particles swollen with a liquid cell growth medium to form a granular gel yield stress material which undergoes a phase transformation from a solid phase to a liquid-like phase when an applied stress exceeds the yield stress. Cells may be placed in the three-dimensional cell growth medium according to any shape or geometry, and may remain in place within the three-dimensional cell growth medium.

Growth media for three-dimensional cell culture

Embodiments of the present disclosure provide for lubricious structures (also referred to simply as 'structure'), methods of making lubricious structures, methods of using lubricious structures, and the like.

Lubricious structures, methods of making them, and methods of use

Described herein are various embodiments of a valve that may be opened and closed using a thixotropic or 'stress yield' material, or other material that temporarily changes phase upon application of energy to the material. More particularly, some embodiments may include a valve that is opened and closed using a granular gel that is a temporary phase change material.

Valve incorporating temporary phase change material

Disclosed herein is a bioreactor system that allows perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprises a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. The system can further comprise an absorbant material that creates an osmotic pressure gradient to produce perfusive flow. In some embodiments, osmotic pressure draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.

Perfusion bioreactor driven by osmotic pressure gradients

A method or apparatus for creating a three-dimensional tissue construct of a desired shape for repair or replacement of a portion of an organism. The method may comprise injecting at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The apparatus may comprise an injector configured to inject at least one biomaterial in a three-dimensional pattern into a first material such that the at least one biomaterial is held in the desired shape of the tissue construct by the first material. The first material may comprise a yield stress material, which may be a material exhibiting Herschel-Bulkley behavior. The tissue construct may have a smallest feature size of ten micrometers or less.

High speed 3D printing system for wound and tissue replacement

An organic microgel system as support material for 3D printing of soft materials such as silicone and methods for manufacturing and using the organic microgel system are disclosed. In some embodiments, the organic microgel system comprises a plurality of microgel particles formed by blending a di-block copolymer and a tri-block copolymer in an organic solvent. The organic microgel system may allow high precision 3D printing of silicone objects with complex shapes.

Organic microgel system with di&#x2014;and tri-block blends of herschel-bulkley fluid for 3D printing of sacrificial support structures

A method or apparatus for three-dimensionally printing. The method may comprise causing a phase change in a region of the first material by applying focused energy to the region using a focused energy source, and displacing the first material with a second material. The apparatus may comprise a container configured to hold a first material, a focused energy source configured to cause a phase change in a region of the first material by applying focused energy to the region, and an injector configured to displace the first material with a second material. The first material may comprise a yield stress material, which is a material exhibiting Herschel-Bulkley behavior. The yield stress material may comprise a soft granular gel. The second material may comprise one or more cells.

3D printing using phase changing materials as support

Described herein are methods and apparatus for constructing tissue replacements, such as bone replacements that may be used to repair damaged or missing segments of bone, such as may occur in wound repair or as a repair of a congenital anomaly. These methods involve a three-dimensional (3D) cell growth medium made from a yield stress material that allows cells and structures to be easily deposited and positioned.

Fabrication of autologous bone

Disclosed herein is an integrated assay system that can be used, for example, to monitor and screen cells in 3D culture. This system involves a 3D cell growth medium made from a yield stress material that allows cells to be deposited, e.g. by 3D printing, samples to be taken, and the extracellular environment manipulated.

3D micro fabrication and screening

3D printing using phase changing matertials as support

Organic microgel system for 3D printing of silicone structures

Growing community of inventors

Gainesville, FL, United States of America

Thomas Ettor Angelini