University System of Maryland

Thomas E Winkler

Gregory F Payne

Reza Ghodssi

Eunkyoung Kim

Deanna L Kelly

William E Bentley

George Banis

Christopher Kitchen

Hadar Ben-Yoav

Mijeong Kang

Methods are provided that allow global access to redox-based molecular information by coupling electrochemical measurements with signal processing approaches. More specifically, the disclosure provides methods that rely on the use of redox probes to assay samples for redox activities that act to exchange electrons with the probe thereby generating detectable optical and electrochemical signature signals that can then be assigned to a sample feature of interest. In particular embodiments, the disclosed assay methods are useful for diagnosis and prognosis of disorders, such as schizophrenia, that are found to be associated with a specific redox-based signature within a subject sample.

Redox probing for chemical information

A lab on a chip device includes a whole blood inlet port and microchannels to transport a whole blood sample or plasma skimmed from the whole blood sample into a detection chamber that includes at least one 3-electrode set of a counter electrode, a working electrode and a reference electrode. The counter electrode, the working electrode and the reference electrode may present bare, unmodified surfaces that are disposed so that clozapine present in the whole blood sample is detected via a reduction-oxidation reaction. Alternatively, the working electrode surface may include catechol grafted to chitosan. A method of detecting analytes and biomarkers includes collecting a whole blood sample, loading the sample into a point-of-care testing (POCT) device that includes at least one working electrode; testing the sample for the occurrence of a redox reaction; and calculating the total oxidative charge when the working electrode is bare or modified as before.

Analytical micro-devices for mental health treatment monitoring

Growing community of inventors

Greenbelt, MD, United States of America

Thomas E Winkler