Harvard College

Charité – Universitätsmedizin Berlin

Thomas E Schaus

Peng Yin

Jocelyn Yoshiko Kishi

Nikhil Gopalkrishnan

David Y Zhang

Wei Sun

Feng Xuan

Sungwook Woo

David J Mooney

Xi Chen

Georg N Duda

Steven Henry Strassmann

Jongmin Kim

Youngeun Kim

Pascal Joly

Anke Dienelt

Andrea Sass

The present disclosure provides, in some aspects, nucleic acid-based molecular tools that enable the recording of molecular structure and soluble signals as well as the programmed assembly of molecular structures.

Molecular programming tools

Provided herein, in some embodiments, are molecular authentication methods, systems, and compositions.

Methods and compositions for molecular authentication

Provided herein, in some aspects, are methods of imaging molecules without a microscope or other specialized equipment, referred to herein as 'microscope-free imaging (MFI).' Herein, 'molecular instruments' (e.g., DNA-based and protein-based molecules) are used, instead of microscopes, in a &#x201c;bottom-up&#x201d; approach for inspecting molecular targets.

Microscope-free imaging

Provided herein are molecular verification (authentication) systems, methods and compositions that use molecular (DNA/RNA) circuitry to enable specific molecularly encrypted solutions.

Molecular verification systems

The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.

Sequence design for efficient assembly of nucleic acid structures

The present invention is directed to a device for enriching cells with a cell surface marker, comprising an aptamer suitable for specifically binding the cell surface marker, and beads coupled thereto, wherein the aptamer is coupled to the beads in a manner that allows for release of cells expressing the cell surface marker, in the absence of a chemical agent, and production of a cell population enriched for cells expressing the cell surface marker, substantially free of beads and aptamer. Kits comprising the device or components thereof, and methods of cell enrichment, are also provided. In exemplary embodiments, the device contains an aptamer that specifically binds CD31.

Compositions and methods for enrichment of cells

The invention provides systems and methods for spatial sequestration of elements in nucleic acid circuits.

Spatial sequestration of dynamic nucleic acid circuits

The invention provides compositions and methods relating to self-assembly of structures of various size and shape complexity. The composition include synthetic single-stranded polymers having a backbone and pre-determined linear arrangement of monomers.

Growing community of inventors

Arlington, MA, United States of America

Thomas E Schaus