Life Technologies Corporation

Thomas Diller

Kyle Bulloch

Xin Mathers

Timothy Updyke

Jennifer Miller

Robert Bennett

Siddharth Kannan

George Hanson

Bethany Sutton

Joseph Amshey

Regina Rooney

Siddarth Kannan

Timothy Vale Updyke

Provided herein is an apparatus for gel electrophoresis comprising a cassette and a comb having at least one wedge-shaped tooth.

Electrophoresis gel cassette and comb

Electrophoresis gel cassette

Electrophoresis slab gel sample loading guide

Electrophoresis cassette and sample loading guide

Cassette support base for electrophoresis gel casting

An apparatus for preparation of an electrophoresis slab gel may include a base having an opening configured to receive a cassette configured to contain an electrophoresis slab gel, a clamping mechanism configured to move relative to the base between an open position in which the clamping mechanism permits insertion of a cassette into the base, and a closed position in which the clamping mechanism is configured to clamp a cassette received in the base, a compressible pad operatively coupled to the clamping mechanism in a position to compress against a cassette received in the base in the closed position of the clamping mechanism. The cassette may include a first plate and a second plate, and a spacer mechanism separate from each of the first and second plates, the spacer mechanism configured to be positioned between the inner faces of the first plate and the second plate. When subjected to a clamping force exerted on the outer faces of the first and second plates, the spacer mechanism is configured to maintain a separation distance between the inner faces of the first and second plates, and provide a seal to prevent leakage of an electrophoresis gel solution introduced between the plates.

Preparation of electrophoresis gels, and related devices, systems, and methods

Electrophoresis cassette with sample loading guide

Rig for electrophoresis gel casting

The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

Rapid protein labeling and analysis

Growing community of inventors

San Diego, CA, United States of America

Thomas Diller