Inbiose N.v.

Thomas Decoene

Joeri Beauprez

Annelies Vercauteren

Pieter Coussement

Gert Peters

Tom Verhaeghe

Sofie Aesaert

Kristof Vandewalle

Nausicaä Lannoo

The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The present invention relates to newly identified sialyltransferases having alpha-2,3-sialyltransferase activity on the galactose residue of lactose. The invention also describes methods for the production of a 3'sialylated oligosaccharide using any one of the newly identified sialyltransferases as well as the purification of the 3′sialylated oligosaccharide. The present invention also provides a cell for production of the 3′sialylated oligosaccharide and the use of the cell in a cultivation or incubation.

Sialyltransferases for the production of sialylated oligosaccharides

The disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of cultivation or fermentation of metabolically engineered cells. The disclosure describes a cell metabolically engineered for production of a mixture of at least four different neutral non-fucosylated oligosaccharides. Furthermore, the disclosure provides a method for the production of a mixture of at least four different neutral non-fucosylated oligosaccharides by a cell as well as the purification of at least one of the oligosaccharides from the cultivation.

Production of a mixture of neutral non-fucosylated oligosaccharides by a cell

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Zwijnaarde, Belgium

Thomas Decoene