Takara Bio Usa, Inc.

Cholestech Corporation

Thomas David Schaal

Maithreyan Srinivasan

Jude Menlo Dunne

Alain-Albert Mir

Chun-Wah Lin

Shanavaz Loharasp D Nasarabadi

Suyue Qian

Shireen S Khan

Patricio Espinoza

George E Withers, Iii

Greg Bennett

Alain Mir

Jeff Shindelman

Eileen Gee

Patricio A Espinoza-Vallejos

William H Chapman, Jr

George E Iii Withers

The present disclosure provides methods, compositions, and systems employing blocked primers. Aspects of the disclosure include providing a blocked primer reaction mixture that includes a blocked primer and a template nucleic acid component from a single cell; unblocking the blocked primer to produce an active primer reaction mixture and subjecting the activated primer reaction mixture to primer extension conditions, such as nucleic acid amplification conditions.

Methods and compositions employing blocked primers

The present disclosure provides methods, device, assemblies, and systems for dispensing and visualizing single cells. For example, provided herein are systems and methods for dispensing a dispense volume into a plurality of wells of a multi-well device, where, on average, a pre-determined number of cells (e.g., 1-20) are present in the dispense volume, and determining, via a cellular label, the number of cells present in each of the plurality of wells. Such dispensing and cell detection may be repeated a number of times with respect to wells identified as having less than the pre-determined number of cells in order increase the number wells in the multi-well device containing the desired number (e.g., a single cell).

Method for rapid accurate dispensing, visualization and analysis of single cells

Provided herein are systems and methods for whole genome amplification and sequencing. In particular, provided herein are systems and methods for detection of nucleic acid variants (e.g., rare variants) in limited samples.

Systems and methods for whole genome amplification

An assay device and method for determining the concentration of low density lipoprotein (LDL)-associated cholesterol (LDL-C) in a body-fluid sample, such as a blood sample is described.

Device and method for measuring LDL-associated cholesterol

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San Francisco, CA, United States of America

Thomas David Schaal