Mycogen Plant Science, Inc.

Other

Thomas D Sullivan

Alan H Christensen

Peter H Quail

Howard P Hershey

Robert A Sharrock

Donald B Stewart

An attention performance test uses a visual display, a test subject input device, a memory and a programmed processor coupled to each of the above to administer a test to a test subject. A test target, which for example can be a word colored in a first color but meaning another color, is attempted to be matched by the test subject to one of a series of sequentially presented visual response targets. In one embodiment, a nonvisual test stimulus is also provided to the test subject, the test subject having previously been instructed to respond to the nonvisual test stimulus (such as a beep) in a particular way. The test also measures the response latency of the test subject, i.e., whether the test subject has assumed a correct position for entering a response. The responses from the test subject are recorded and may be compared with data derived from a test subject database in assessing whether an attention deficit disorder exists.

Measurement of attention span and attention deficits

A DNA segment from the upstream untranscribed region of a maize ubiquitin gene is disclosed. This ubiquitin promoter region, which comprises heat shock consensus elements, initiates and regulates the transcription of genes placed under its control. Recombinant DNA molecules are also described in which a ubiquitin promoter is combined with a plant expressible structural gene for regulated expression of the structral gene and for regulated control of expression when stressed with clevated temperatures. Such recombinant DNA molecules are introduced into plant tissue so that the promoter/stuctural gene combination is expressed.

Plant ubiquitin promoter system

A method of inducibly enhancing the constitutive expression of a DNA sequence of interest is described in which plant cells are transformed with a DNA sequence of interest that is operably joined to a plant ubiquitin regulatory region comprised of a heat shock element, a promoter, a transcription start site, an intron and a translation start site, When monocot or dicot plant cells are subjected to permissive heat shock temperatures, the level of expression of the DNA sequence of interest is enhanced.

A dark- and light-regulated chlorophyll a/b (Cab) binding protein promoter/regulatory system from maize is disclosed. This promoter/regulatory system is capable of accounting for as much as 80% of the total Cab mRNA in the dark and results in enhancement of expression from 3- to 6-fold after conditions of illumination. This Cab promoter/regulatory system may be recombined with structural genes for expression in plant cells.

Dark and light regulated chlorophyll A/B Binding Protein

A plant chlorophyll a/b binding (Cab) protein gene was isolated from a maize genomic library. The promoter/regulatory system of this gene actively functions in controlling Cab gene expression under conditions of darkness and in enhancing gene expression under conditions of light. This invention contemplates the utilization of the Cab promoter/regulatory system in regulating non-homologous structural gene expression in transgenic plants under conditions of darkness followed by further enhancement of gene expression under conditions of light.

Dark and light regulated chlorophyll A/B binding protein

A method of inducibly enhancing the constitutive expression of a DNA sequence of interest is described in which plant cells are transformed with a DNA sequence of interest that is operably joined to a plant ubiquitin regulatory region comprised of a heat shock element, a promoter, a transcription start site, an intron, and a translation start site. When monocot or dicot plant cells are subjected to permissive heat shock temperatures, the level of expression of the DNA sequence of interest is enhanced.

A DNA segment from the upstream untranscribed region of a maize ubiquitin gene is disclosed. This ubiquitin promoter region, which comprises heat shock consensus elements, initiates and regulates the transcription of genes placed under its control. Recombinant DNA molecules are also described in which a ubiquitin promoter is combined with a plant expressible structural gene for regulated expression of the structural gene and for regulated control of expression when stressed with elevated temperatures. Such recombinant DNA molecules are introduced into plant tissue so that the promoter/structural gene combination is expressed.

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Madison, WI, United States of America

Thomas D Sullivan