Columbia University

Thomas C Wright, Jr

Saul J Silverstein

Octavian Lungu

Ralph M Richart

This invention provides a method of detecting a high oncogenic-risk type human papillomavirus in a subject which comprises: obtaining from a subject a specimen containing cervical cells and treating the specimen so as to recover nucleic acid molecules present in the cervical cells; contacting the resulting nucleic acid molecules with multiple pairs of single-stranded labeled oligonucleotide primers capable of specifically hybridizing with a different high oncogenic-risk type of human papillomavirus; amplifying any nucleic acid molecules to which a pair of primers hybridizes so as to obtain a double-stranded amplification product and treating any double-stranded amplification product so as to obtain single-stranded nucleic acid molecules; contacting any resulting single-stranded nucleic acid molecules with multiple single-stranded labeled oligonucleotide probes which are capable of specifically hybridizing with such high oncogenic-risk types of human papillomavirus; contacting any resulting hybrids with a marked antibody capable of specifically forming a complex with the labeled probe, when the probe is present in such a complex; and detecting the presence of any resulting complexes, the presence thereof being indicative of the presence of a high oncogenic-risk type human papillomavirus in the initial specimen.

Detection of high oncogenic-risk papilloma virus in high grade cervical

This invention provides a method of typing a human papillomavirus in a patient infected by human papillomavirus which comprises: obtaining a sample containing DNA from the human papillomavirus to be typed; amplifying the L1 portion of the human papillomavirus DNA; treating the resulting amplified DNA with a plurality of predetermined restriction enzymes so as to produce restriction fragments; and analyzing the fragments so produces so as to type the human papillomavirus.

Polymerase chain reaction/restriction fragment polymorphism method for

The subject invention provides a method of diagnosing congenital adrenal hyperplasia in a human subject. The subject invention also provides a method of typing a human papillomavirus in a patient infected by a human papillomavirus. The subject invention further provides a method for detecting Mycobacteria in a clinical sample. Finally, the subject invention provides a method for typing Mycobacteria in a clinical sample containing Mycobacteria.

Growing community of inventors

Irvington, NY, United States of America

Thomas C Wright, Jr