Novo Nordisk Healthcare Ag

Novo Nordisk A/s

Thomas Budde Hansen

Arne Staby

Steffen Kidal

Janus Krarup

Haleh Ahmadian

Susanne Bang

Are Bogsnes

Daniel E Rasmussen

Anne Charlotte Arentsen

Ernst Broberg Hansen

Camilla Kornbeck

Nina Johansen

Lene Vædele Madsen Tomoda

Turid Preuss

Erik Halkjær

Jesper Christensen

Charlotte Schou Hunneche

Erik HALKJÆR

The invention relates to methods for obtaining a protein conjugate wherein a cysteine residue of the protein serves as attachment point for the chemical moiety conjugated to the protein.

Method for thioether conjugation of proteins

Described are methods for determination of peptide specific parameter(s) of a mixture comprising a target peptide and a related impurity (or impurities) to be used in a simulation model of chromatographic separation using mathematical model(s). Also described are chromatographic simulation methods using above determined parameters, as well as computer systems and computer programs for performing one or more of the above method(s).

Methods of optimizing chromatographic separation of polypeptides

The present invention relates to Vitamin K-dependent protein compositions having a very low, or negligible, content of protein contaminants. The present invention also relates to method applicable in the preparation of such Vitamin K-dependent protein compositions. Such methods may either be used alone or in sequential combination with the purpose of reducing the relative content of protein contaminants. The present invention is particularly relevant in the preparation of compositions of coagulation factors selected from Factor X polypeptides (FX/FXa), Factor IX polypeptides (FIX/FIXa), Factor VII polypeptides (FVII/FVIIa), and the anticoagulant Protein C, in particular Factor VII polypeptides.

Reduction of the content of protein contaminants in compositions comprising a vitamin K-dependent protein of interest

The present invention relates to a novel method for improving the viral safety of liquid Factor VII compositions, in particular those comprising active Factor VII polypeptides (a Factor VIIa polypeptide).

Virus filtration of liquid factor VII compositions

Method for purifying a racemized polypeptide by ion-exchange chromatography.

Separation of polypeptides comprising a racemized amino acid

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Copenhagen, Denmark

Thomas Budde Hansen