Inguran, LLC

Xy, Inc.

Chd Bioscience, Inc.

Cdh Bioscience, Inc.

Chata Biosystems, Inc.

Thomas Boyd Gilligan

Kenneth Michael Evans

Edwin Dean Neas

John Louis Schenk

Ramakrishnan Vishwanath

Clara Gonzalez-Marin

Jerald Edward Kuiken

Richard W Lenz

Johnathan Charles Sharpe

Juan F Moreno

Sam Hogue

Tae Kwang Suh

Kilby Willenburg

Richard Lenz

Todd A Cox

Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.

Methods of processing sperm for sex sorting

The invention provides serialized artificial insemination straws, as well as systems and methods for authentication of the serialized artificial insemination straws.

Serialized artificial insemination straws and systems and methods of authentication

Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.

High pressure sperm sorting and flow cytometer methods

The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.

Sperm nuclei and methods of their manufacture and use

The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.

Low sugar sperm media and compositions

Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.

Sperm processing method, apparatus and related media compositions

A fluid handling and delivery system useful in generating a fluid stream in the flow path of microfluidic device.

Generating a fluid stream in a microfluidic device

This disclosure relates to cell sorting methods, and particularly cell sorting methods that improve the efficiency or productivity of sorting in a particle sorting instrument utilizing a measured parameter of sorting efficiency. In one embodiment, minimum productivity and minimum purity may be established and maintained while attempting to maximize the sorting efficiency. While in another embodiment, a minimum sorting efficiency and a minimum purity may be established and maintained while attempting to maximize the productivity of a sort.

High efficiency methods of sex sorting sperm

Compositions () and methods of using such compositions () to condition, clean, or disinfect the flow path of a conduit () of a microfluidic devices (), such as flow cytometers or liquid chromatographs.

Antimicrobially active compositions

Storage cane

A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.

Sperm staining and sorting methods

Antimicrobial compositions and methods of using such antimicrobial compositions to provide a reduction in populations of viable microorganisms.

Differential evaporation potentiated disinfectant system

Generally, polymeric members and laser marking methods for producing visible marks on polymeric members, such as on thin and/or curved surfaces. The laser marking methods can include methods of laser marking straws with the step of matching laser source properties to the properties of straws being marked or with the step of laser marking straws having photochromic dyes.

Growing community of inventors

College Station, TX, United States of America

Thomas Boyd Gilligan