Carl Zeiss Microimaging Gmbh

Thomas Bocher

Renhu Shi

Werner Kleinschmidt

Ingo Fahlbusch

Andreas Nolte

Anke Vogelgsang

A microscope for observing an object selectably using the bright-field transmitted light contrast procedure or the incident-light fluorescence contrast procedure. During the bright-field transmitted light contrast procedure, an illumination beam path is directed from a bright-field light source to the object and an imaging beam path is directed from the object through the microscope objective into the microscope tube, which includes a fluorescence unit that includes a fluorescence excitation light source, an illumination optical system, a filter set having at least one excitation filter and at least one emission filter, as well as a beamsplitter. The fluorescence unit is mounted movably, so that in a first end position of movement the beamsplitter and the emission filter are out of the imaging beam path of the microscope and in a second end position of movement they are in the imaging beam path between the microscope objective and the microscope tube.

Microscope for observing a sample in the bright field illumination by transmitted light or in fluorescence-contrast epi-illumination

The invention relates to a microscope objective with preferably anti-symmetric lenses or lens groups with an optical magnification of &#x2212;100 and a visual field factor of 20. According to the invention the microscope objective consists of 9 lenses with 3 cemented elements, starting from the object side (left), a lens which is almost a hemisphere Lwith positive refractive power, a meniscus lens Lwith positive refractive power, a two-part cemented element Gwith positive refractive power, another two-art cemented element Gwith positive refractive power, a two-part cemented element Gwith negative refractive power, and finally a meniscus lens Lwith negative refractive power. By using cemented elements and lens pairings of the same construction, production costs can be reduced compared with methods of the prior art while image contrast is improved.

Microscope objective

A microscope objective having at least four lenses or groups of lenses and which can be used to improve image contrast. According to the invention, a phase plate, aligned concentrically to the optical axis, can be integrated into and taken out of the air space between the first lens and the second lens, as viewed from the object side. The defined arrangement of the phase plate and the associated shift of the real pupil into the air space between the first two lenses or groups of lenses, respectively, of the microscope objective allows a microscope objective, initially designed as a bright-field variant, to be redesigned as a phase contrast variant with relative ease.

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Goettingen, Germany

Thomas Bocher