Gen-probe, Inc.

Thomas B Ryder

Sherrol Hoffa McDonough

Daniel L Kacian

Nanibhushan Dattagupta

Yeasing Y Yang

Elizabeth R Billyard

Philip W Hammond

Diane L McAllister

Richard C Harvey

Nancy Lau Shen

Frank R Gonzales

Maria R Castillo

Karen W Shannon

Keiichi Kamisango

Theodore Jesse Kwoh

A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

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Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type 1 from other viruses.

Detection of human immunodeficiency virus type 1

Amplification oligonucleotides and hybridization assay probes which distinguish Human Immunodeficiency Virus type from other viruses.

A method for amplification of a nucleic acid strand in a test sample. The method includes contacting the nucleic acid strand from the test sample simultaneously with at least three oligonucleotide primers. At least one primer is a promoter-primer, and at least one other primer is complementary to the nucleic acid strand, and one other primer is complementary to a strand complementary to the nucleic acid strand. The method further includes contacting the nucleic acid strand and primers with one or more proteins having RNA-directed and/or DNA-directed DNA polymerase activities, an RNA polymerase activity, and an RNAse H activity under primer-extension conditions to allow amplification of a target region in the nucleic acid strand at essentially constant temperature.

Methods of enhancing nucleic acid amplification

Methods of amplifying nucleic acids using promoter-containing primer

The present invention concerns the use of oligonucleotides to inhibit propagation of human immunodeficiency virus (HIV). Preferred HIV target sites are identified and oligonucleotides designed to hybridize to a target site, or be analogous to a target site, are described. The preferred use of the oligonucleotides is to inhibit HIV propagation in a patient infected with HIV.

Oligonucleotides and methods for inhibiting propagation of human

Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducability. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.

Methods for determining pre-amplification levels of a nucleic acid

Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducability. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.

Kits for determining pre-amplification levels of a nucleic acid target

Method and kit for the preparation of nucleic acid, such as for amplification. The nucleic acid may be prepared by centrifuging a sample of whole cells, particularly mononuclear cells, with an appropriate centrifugation medium so as to separate desired cells from non-desired cells, then removing and lysing said desired cells. A complexing agent able to complex ferric ion may be added to the lysed cells. The nucleic acid associated with the lysed cells can be amplified, and the nucleic acid can also be screened for a certain nucleic acid sequence, such as a vital nucleic acid sequence.

Amplification of nucleic acids from mononuclear cells using iron

Method for destroying the ability of a nucleic acid to be amplified, comprising the step of contacting the nucleic acid with bleach in an amount and for a time sufficient to inhibit the ability of that nucleic acid to be amplified in an amplification reaction.

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Escondido, CA, United States of America

Thomas B Ryder