Basf Aktiengesellschaft

Basf Se Corporation

Evonik Degussa Gmbh

Omnigene Bioproducts, Inc.

Other

Thomas A Patterson

Janice G Pero

R Rogers Yocum

Theron Hermann

Oskar Zelder

Hartwig Schröder

Stefan Haefner

Corinna Klopprogge

Andrea Herold

Mark K Williams

R Roger Yocum

Kai-Uwe Baldenius

Christoph Wittmann

Christine Beck

Elmar Heinzle

Jens Kroemer

Theron Herman

Rogers R Yocum

Hartwig SCHRÖDER

Mark Williams

The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate &#x3b1;-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.

Methods and microorganisms for production of panto-compounds

The present invention relates to microorganisms and methods for producing methionine by reactivation of the MetH enzyme.

Microorganisms with a reactivation system for cob(I)alamin-dependent methionine synthase

The present invention relates to microorganisms and processes for the efficient preparation of L-amino acids such as L-methionine. In particular, the present invention relates to microorganisms and processes in which the formation and/or accumulation of homolanthionine in the methionine pathway is reduced and/or prevented.

Microorganism and process for the preparation of L-methionine

The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

Microorganisms and processes for enhanced production of pantothenate

The present invention features methods for the identification of compounds and compositions useful as antibiotics and antibacterial agents. In particular, the invention features methods for the identification of modulators of a previously unidentified target protein, termed CoaX. High-throughput assay systems are featured as well as assay kits for the identification of CoaX modulators. Also featured are coaX nucleic acid molecules and purified CoaX proteins, as well as recombinant vectors and microorganisms including the gene, coaX.

Microorganisms and assays for the identification of antibiotics

The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate by enzymes that contribute to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

The present invention features improved methods for producing pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities. In particular, the invention features methods for reducing byproduct formation and increasing yields and purity of desired product. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

Processes for enhanced production of pantothenate

A lifting dolly is provided for quickly and easily lifting a helicopter or the like while on the ground and for transporting the helicopter for maintenance or storage purposes. The lifting dolly comprises a relatively low profile central frame supported for rolling movement by a pair of wheel assemblies and including laterally outwardly pivoting lifting arms for engaging the skid tubes of a helicopter. In use, the dolly is positioned beneath the helicopter and the frame is lowered to rest upon the ground whereupon the lifting arms are pivoted outwardly to press wedge-shaped lifting fingers beneath the skid tubes. Rollers on the underside of the frame permit lateral frame shifting as the fingers engage the skid tubes such that the dolly centers itself between the skid tubes. When the skid tubes are engaged, the frame is elevated by the wheel assemblies to lift the helicopter for convenient rolling movement.

Lifting dolly for helicopters

Growing community of inventors

North Attleboro, MA, United States of America

Thomas A Patterson