Bayer Healthcare Aktiengesellschaft

Bayer Aktiengesellschaft

Thoams Krahn

Delf Schmidt

Martin Bechem

Wolfgang Paffhausen

Andreas Schade

A process for the quantitative optical analysis of fluorescently labeled biological cells involves contacting a cell layer on a transparent support at the bottom of a reaction vessel with a solution containing the fluorescent dye. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved.

Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays

In a process for the quantitative optical analysis of fluorescently labelled biological cellsa cell layer on a transparent support at the bottomof a reaction vesselis in contact with a solutioncontaining the fluorescent dyeThe sensitivity of analytical detection can be considerably improved if to the fluorescent dyealready present in addition a masking dyewhich absorbs the excitation lightfor the fluorescent dyeand/or its emission lightis added to the solutionand/or if a separating layerpermeable to the solution and absorbing and/or reflecting the excitation lightor the emission lightis applied to the cell layer at the bottomThis process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layermust in this case be composed such that it has a high power of reflection for the luminescent light. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layerat the bottomof a reaction vesselis in contact with a solution (supernatant) in which a fluorescent or luminescent ligandis dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dyewhich absorbs the excitation lightfor the fluorescent dye and/or its emission light or (in the case of luminescent ligands) the luminescent light is added to the supernatantInstead of the masking dye in the solutionor optionally as an additional measure, a separating layerpermeable to the solutionand absorbing and/or reflecting the excitation lightand/or the emission light or the luminescent light can be applied to the cell or receptor layerat the bottom

Masking background flourescence and luminescence in optical analysis of biomedical assays

In a process for the quantitative optical analysis of fluorescently labelled biological cells , a cell layer on a transparent support at the bottom of a reaction vessel is in contact with a solution containing the fluorescent dye . The sensitivity of analytical detection can be considerably improved if to the fluorescent dye already present in addition a masking dye , which absorbs the excitation light for the fluorescent dye and/or its emission light , is added to the solution and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to the cell layer at the bottom . The separating layer must be composed such that it has a high power of reflection for the luminescent light . Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dye which absorbs the excitation light for the fluorescent dye and/or its emission light or the luminescent light is added to the supernatant . Instead of the masking dye in the solution or optionally as an additional measure, a separating layer permeable to the solution and absorbing and/or reflecting the excitation light and/or the emission light or the luminescent light can be applied to the cell or receptor layer at the bottom

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Hagen, Germany

Thoams Krahn