Mycogen Corporation

Phytogen

J. G. Boswell Company

Novartis Finance Corporation

Thirumale S Rangan

David M Anderson

Kanniah Rajasekaran

Richard L Yenofsky

Richard Lorne Hudspeth

John W Grula

Miriam Fine

Nadine B Carozzi

Richard Yenofsky

Richard Lotstein

Douglas Rice

Methods of producing pesticidal cotton cells, cotton plants and seeds by transformation with heterologous pesticidal lectin encoding nucleic acid sequences are disclosed.

Methods for producing pesticidal cotton plants expressing lectins

A transformed cotton plant. The transformed cotton plant comprises DNA derived from a source other than cotton plants, wherein the DNA, when transformed into the cotton plants, confers a phenotype not expressed inn a parent cotton.

Transformed cotton plants

Chimeric genes encoding lectins exhibiting pesticidal activity (for example, insecticidal and/or fungicidal activity) are disclosed which can be used to transform cotton to yield cotton cells, plants, and seeds in which the chimeric genes are expressed. Such transformed cotton cells are pesticidal when ingested by cotton pests.

Cotton cells, plants, and seeds genetically engineered to express insecticidal and fungicidal chitin binding proteins (lectins)

A transformed cotton plant. The transformed cotton plant comprises DNA derived from a source other than cotton plants, wherein the DNA, when transformed into the cotton plants, confers a phenotype not expressed in a parent cotton.

A method for producing transformed cotton plants. The method comprising providing cotton explants, incubating the cotton explant in the presence of a vector comprising a selectable marker to produce treated explants, growing the treated explants to produce callus and selecting transformed callus.

Transformation of cotton plants

A vector for transforming cotton. The vector comprising integration sequences for integrating into the genome of cotton plants, a promoter for promoting transcription in cotton plants, a DNA sequence encoding a selectable marker and a termination signal for terminating transcription in cotton plants.

A promoter isolated from a cotton gene encoding the small subunit of ribulose bisphosphate carboxylase is described. The isolated promoter is operably linked to a coding sequence of interest to make a chimeric gene.

Cotton promoter

A method for producing somaclonal variant cotton plant. The method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.

Method for producing somoclonal variant cotton plants

A somaclonal variant cotton plant. The somaclonal cotton plant is produced by a method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.

Cotton somaclonal variants

A method for producing somaclonal variant cotton plant. The method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.

Method for producing somaclonal variant cotton plants

A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.

Regeneration of cotton plant in suspension culture

There are provided methods for regenerating cotton by tissue and suspension culture starting with explants which are the hypocotyl, cotyledon or immature embryos. This also taught methods to transform cotton and improve cotton by selective growth.

Growing community of inventors

San Dimas, CA, United States of America

Thirumale S Rangan