Dsm IP Assets B.v.

Holland Sweetener Company, V.o.f.

Isobionics B.v.

Theodorus Sonke

Peter Jan Leonard Mario Quaedflieg

Hendrik Jan Bosch

Petronella Catharina Raemakers-Franken

Wilhelmus Hubertus Joseph Boesten

Martinus Julius Beekwilder

Peter J L M Quaedflieg

Friso Bernard Jan Van Assema

Pieter Grijpstra

Adolf F V Wagner

Adolf Fritz Volker Wagner

Adolf Fritiz Volker Wagner

Rudolf Van Der Pol

Cornelis Gerrit Nijs Korevaar

Renate Francisca Tandler

Gerrit Jan Willem Euverink

Hendrik Jan Bouwmeester

Jihane Achkar

Rene M De Jong

The present invention relates to a valencene synthase, to a nucleic acid encoding such valencene synthase, to a host cell comprising said encoding nucleic acid sequence and to a method for preparing valencene, comprising converting farnesyl diphosphate to valencene in the presence of a valencene synthase according to the invention.

Valencene synthase from callitropsis nootkatensis

The invention relates to isolated polypeptides having &#x3b1;-H-&#x3b1;-amino acid amide racemase activity and nucleic acids encoding the same. The invention also relates to vectors and host cells comprising the nucleic acids according to the invention. The invention also relates to methods of producing and using the polypeptides according to the invention. The invention also relates to a method for isolating polypeptides having &#x3b1;-H-&#x3b1;-amino acid amide racemase activity, for isolating nucleic acids encoding the same and for isolating microorganisms comprising polypeptides having &#x3b1;-H-&#x3b1;-amino acid amide racemase activity. The invention also relates to new microorganisms comprising polypeptides having &#x3b1;-H-&#x3b1;-amino acid amide racemase activity.

Polypeptides having &#x3b1;-H-&#x3b1;-amino acid amide racemase activity and nucleic acids encoding the same

The invention relates to nucleic acid sequence encoding enantioselective amides with an amino acid sequence of SEQ ID NO: 2. The invention also relates to a process for fermentation, comprising a batch and a feed phase, of a microorganism in a fermentation medium, wherein the microorganism expresses a nucleic acid according to the invention and wherein between 0.5 and 50 mg of Zn/ml of fermentation medium is fed during the fermentation. The invention also relates to a process for the preparation of enatiomerically enriched carboxylic acid and/or an enatiomerically enriched carboxylic acid amide, in which a mixture of corresponding D- and L-carboxylic acid amides is contacted with a expression product according to the invention in the presence of 0.01 mM&#x2013;100 mM Zn, whereby one of the enantiomers of the carboxylic acid amide is enatiomerically hydrolysed to from corresponding enatiomerically enriched carboxylic acid, while the other enantiomer of the carboxylic acid amide remains unchanged.

Nucleic acid sequences encoding enantioselective amidases

A process for preparation of a compound with enhanced optical purity is disclosed wherein a mixture of the enantiomers of a chiral amine is brought into contact with an enzyme having peptide deformylase activity with a bivalent metal ion as a cofactor.

Process for the preparation of compounds with enhanced optical purity

Process for the preparation of a compound with enhanced optical purity wherein a mixture of the enantiomers of a chiral compound of formula 1wherein: Preferably the peptide deformylase is chosen from the class EC 3.5.2.27 or EC 3.5.1.31, and contains the sequences of (I) HEXXH, (ii) EGCLS and (iii) GXGXAAXQ. The bivalent metal may be chosen from the group of Fe, Ni, Mn and Co, preferably Ni or Fe.

The invention relates to the synthesis of aspartame involving enzymatic deformylation of an N-formyl-&agr;-L-aspartyl-L-phenylalanine compound by treatment with an enzyme having formylmethionyl peptide deformylase activity and having as a co-factor group 5 to 11 bivalent metal ions. The invention also relates to selective preparation and recovery of aspartame from a mixture of N-formyl-&agr;- and &bgr;-L-aspartyl-L-phenylalanine compounds by treatment with such enzyme. And finally, the invention relates to one-pot enzymatic synthesis of aspartame from N-formyl-L-aspartic acid and L- or D,L-phenylalanine methyl ester involving an enzymatic deformylation reaction simultaneously with an enzymatic coupling reaction, as well as to one-pot di- or oligopeptide synthesis by simultaneous enzymatic coupling and deformylation reactions in general.

Synthesis and recovery of aspartame involving enzymatic deformylation step

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Guttecoven, Netherlands

Theodorus Sonke