Agilent Technologies, Inc.

Schering Corporation

Merck Sharp + Dohme Corp.

Theodore R Sana

Paul K Wolber

Robert A Kastelein

J Fernando Bazan

Daniel M Gorman

Terrill K McClanahan

Eric M Leproust

Christi L Parham

Jeanine D Mattson

Erin E Murphy

Jeffery Grein

Hirokazu Kurata

Naoko Arai

Chetan Savkoor

Kathleen M Smith

Clotilde S Perbost

Glenda Choate Delenstarr

Michel G M Perbost

Joseph A Hedrick

Jacqueline C Timans

Johannes Eduard Maria Antonius Debets

Nucleic acids encoding mammalian, e.g., primate or rodent, genes, purified proteins and fragments thereof. Antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositions for both diagnostic and therapeutic utilities are provided.

Nucleic acids encoding TNF&#x3b3;

Nucleic acid encoding TNFx protein

Methods and devices for producing a nucleic acid arrays using in situ nucleic acid array synthesis protocols are provided. A feature of certain embodiments of the invention is that control probes are produced in collections of features, e.g., columns, of the arrays that have been selected according to a particular efficient control probe feature/column selection protocol. A feature of certain other embodiments of the invention is that an 'all-bases-all-layers' probe set is produced in at least one of column of the arrays. Also provided are devices configured for use in the subject methods, as well as arrays produced using the subject methods and devices as well as methods for using such arrays.

Nucleic acid array in situ fabrication methods and arrays produced using the same

Methods and apparatus are disclosed for synthesizing a plurality of biopolymers at predetermined feature locations on a surface of a substrate. One or more of the feature locations comprises degenerate biopolymers. One or more biopolymer subunit precursors are added, in multiple rounds of subunit additions, at each of multiple feature locations on the surface to form the plurality of biopolymers on the surface. For each feature location comprising degenerate biopolymers, the biopolymer subunit precursors comprise a mixture of biopolymer subunit precursors for forming the degenerate biopolymers at the feature location.

Methods and apparatus for preparing arrays comprising features having degenerate biopolymers

Nucleic acids encoding mammalian, e.g., rodent, IL-1&#x3b4;, IL-1&#x3b5;, purified IL-1&#x3b4; and IL-1&#x3b5; proteins and fragments thereof. Antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositions for both diagnostic and therapeutic utilities are provided.

Mammalian cytokines; related reagents and methods

The invention provides a composition, kit and method for hybridizing a probe and target at a temperature lower than their standard hybridization temperature. The chemical component added to the composition has a formula R(NH)C&#x2550;O, where R is amino or alkyl. A method for use of the chemical component and composition is also disclosed.

Compositions and methods for optimized hybridization using modified solutions

Composition and method for optimized hybridization using modified solutions

Nucleic acid arrays that have background features, and methods for using the same, are provided. The subject nucleic acid arrays include both hybridization features and background features, where the background features provide a background signal in a hybridization assay that is made up of a feature substrate component, a nucleic acid probe component and a nucleic acid probe non-specific binding component. In practicing the subject methods, the arrays are contacted with a sample and signals are observed for both hybridization features and background features. The background feature signal is then subtracted from the hybridization feature signal to obtain a background corrected hybridization feature signal that is employed as the output of the assay, e.g., to determine the presence, either qualitatively or quantitatively, of the analyte target nucleic acid in the sample. Also provided are kits for use in practicing the subject methods.

Arrays having background features and methods for using the same

Nucleic acids encoding mammalian, e.g., human receptors, purified receptor proteins and fragments thereof. Antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositions for both diagnostic and therapeutic utilities are provided.

Human receptor proteins; related reagents and methods

Growing community of inventors

Santa Clara, CA, United States of America

Theodore R Sana