Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Other

E. I. Du Pont De Nemours

Theodore Mitchell Klein

Rebecca E Cahoon

William James Gordon-Kamm

Keith S Lowe

Dwight T Tomes

Kevin E McBride

John C Sanford

Ning Wang

George J Hoerster

Arthur Weissinger

Anthony John Kinney

Hajime Sakai

Christopher Jay Scelonge

Joanne E Barton

Sonriza Rasco-Gaunt

Xifan Sun

Hochul Yi

Scott V Tingey

Carolyn A Gregory

Jennie Bih-Jien Shen

Ramgopal Nadimpalli

Ajith Anand

Carlos M La Rota

Hyeon-Je Cho

York Moy

Todd J Jones

Stephen M Allen

J Antoni Rafalski

Michael W Lassner

Bo Shen

Omolayo O Famodu

Edgar Benjamin Cahoon

Linda A Castle

Perry G Caimi

Mitchell Christian Tarczynski

Steven Henry Bass

Karen E Broglie

Jonathan E Lightner

Yumin Tao

Jun-Zhi Wei

Leslie T Harvell

William B Allen

Richard W Pearlstein

Olga Nikolaevna Danilevskaya

Jian-Ming Lee

Changjiang Li

Sean M Bertain

Shiv Bahadur Tiwari

Catherine J Thorpe

Michele Morgante

Craig E Hastings

Xinli Emily Wu

Evgueni Ananiev

Jun-zhi Wei

David Mark Shirbroun

Igor Cunha Oliveira

Bing-Bing Wang

Edward Bruggemann

Sabine U Epelbaum

Natalie L Hubbard

Barbara Ann Marie Rosen

Pedro Herman

Maren L Arling

Antoni J Rafalski

Tai Minh

Kevin E Mcbride

Natalie Louise Hubbard

Carolyn Ann Gregory

Barabara Ann Marie Rosen

Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.

Methods and compositions for the introduction and regulated expression of genes in plants

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Methods and compositions for rapid plant transformation

Methods and compositions for-mediated transformation of plants are provided. Methods include but are not limited to using anstrain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include anstrain, transfer DNAs, constructs and/or plasmids. These includestrains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.

Ochrobactrum-mediated transformation of plants

Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for regulatory regions. Phospholipid transfer protein (PLTP) promoters from maize are provided. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence, such as a maize PLTP promoter, disclosed herein. DNA constructs comprising a promoter operably linked to a heterologous nucleotide sequence of interest are also provided.

Embryo-preferredpromoters and methods of use

Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.

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This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.

Plant transcription factors

The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

Gene expression modulating element

This invention relates to an isolated nucleic acid fragment encoding floral development proteins, more specifically FT, TFL or Ap3 homologs. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the floral development proteins, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the FT, TFL or Ap3 homologs in a transformed host cell.

Floral development genes

Growing community of inventors

Wilmington, DE, United States of America

Theodore Mitchell Klein