Advanced Liquid Logic, Inc.

Other

Theodore M Winger

Vamsee K Pamula

Allen E Eckhardt

Ramakrishna Sista

Noel G Harvey

John D Evans

Carrie A Graham

Sanjay Saha

Gajendrasinh Raolji

Tong Wang

Raveendra Dayam

Yalin Xiong

Ning Wu

Gajendrasinh Balvantsinh Raolji

Carrie Graham

A microfluidic device having a substrate with an electrically conductive element made using a conductive ink layer underlying a hydrophobic layer.

Droplet actuator devices comprising removable cartridges and methods

The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.

Enzyme assays on a droplet actuator

Droplet actuator devices and methods

Droplet actuators and techniques for droplet-based assays are provided. A method of conducting an assay comprises: incubating a droplet in oil, the droplet comprising an umbelliferone substrate, a sample potentially comprising an enzyme which cleaves the umbelliferone substrate, and a zwitterionic surfactant; and detecting a signal emitted from the droplet.

Droplet actuators and techniques for droplet-based enzymatic assays

Droplet actuators that include molecular barrier coatings are provided. The molecular barrier coating may be provided atop the conductive layer of the top substrate, atop the droplet operations electrodes of the bottom substrate, or both. Where the conductive layer of the top substrate and/or the droplet operations electrodes of the bottom substrate are formed of an electrically conductive organic polymer, such as poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS), the molecular barrier coating helps to immobilize the contents of the PEDOT:PSS layer. Further, the molecular barrier coating reduces, preferably entirely eliminates, moisture from seeping into the electrically conducting organic polymer. Methods of conducting droplet operations using the disclosed droplet actuators are also provided.

Droplet actuators that include molecular barrier coatings

The invention is directed to droplet actuator devices and assay methods. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate within an aqueous environment in contact with an oil.

Method of hydrolyzing an enzymatic substrate

The invention is directed to droplet actuator devices and assay methods. The method may include, among other things, a method of conducting enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil, the method including incubating a droplet in oil, the droplet preferably comprising an umbelliferone substrate, a sample, and a cyclodextrin compound. The methods may further include a method of substantially eliminating cross-contamination between droplets in enzymatic containing substrate-based bioassays. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the inclusion complex may be formed using cyclodextrins. In yet another embodiment, the invention may provide a method of enhancing hydrolysis of enzymatic substrates. The methods may further include a method of forming an inclusion complex with the substrate for stabilizing the substrate within an aqueous environment, wherein the substrate may be a 4-MU- or HMU containing substrate. The inclusion complex may be formed using cyclodextrin.

Enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil

A microactuator device includes a base with at least one electrode pad and a permeation membrane. Permeation membrane is typically a water-permeable membrane that is able to deform by applying an electric charge to the electrode pad. The actuator device can be incorporated into valve assembly to open and close the valve. The valve assembly can have a reciprocating valve member operated by the deforming of the water-permeable member. Alternatively, the valve assembly can have an opening positioned to cooperate with the water-permeable membrane so that the deformation of the membrane closes the opening.

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Oceanside, CA, United States of America

Theodore M Winger