Beckman Coulter, Inc.

Coulter International Corp.

Theodore J Gerula

Nery Ortiz

Yi Li

Dana B Jacobs

Michael N Elliott

Sandra Socarras

Wayne Goldson

Paul W Price

Penny Whitley

A linearity control system includes a series of linearity control compositions, each thereof includes white blood cell analogs and stabilized red blood cells in a suspension medium. The concentration of the white blood cell analogs in the series of control compositions increases from 0.2&#xd7;10to 800&#xd7;10analogs per microliter, and the concentration of the white blood cell analogs in at least one control composition is greater than 120&#xd7;10analogs per microliter. The stabilized red blood cells facilitate mono-dispersion of the white blood cell analogs in the suspension medium by gently mixing. The control compositions further include platelet analogs, or additionally include reticulocyte and/or nucleated red blood cell analogs. The linearity control system allows the verification of the reportable measurement range and linearity of the measurements of hematology analyzers for white blood cells, red blood cells and platelets in extended concentration ranges.

Hematology linearity control composition, system and method of use

A method of using a reference control for measurement of immature granulocytes on a blood analyzer is disclosed. The method includes analyzing a reference control that contains an immature granulocyte component made of processed red blood cells for simulating immature granulocytes and a mature white blood cell component for simulating white blood cells of a blood sample on a blood analyzer adapted for measurement of immature granulocytes, and reporting the immature granulocyte component and the mature white blood cell component of the reference control.

Method of using a hematology reference control for measurement of immature granulocytes

A reference control composition containing a nucleated red blood cell component and the method of making are disclosed. The reference control composition includes a nucleated red blood cell component made of fixed non-nucleated blood cells and a suspension medium. The non-nucleated blood cell has a natural cell size substantially similar to a size of nucleus of said nucleated red blood cell of said blood sample. The nucleated red blood cell component can be made of equine, ovine, bovine, feline, canine, or porcine red blood cells; and it is substantially free of nucleic acid. The reference control composition can further include a white blood cell component, a red blood cell component, a platelet component, a reticulocyte component, or combinations thereof. Further disclosed are the methods of using the reference control composition for measurement of nucleated red blood cells on a blood analyzer.

Reference control composition containing a nucleated red blood cell component made of non-nucleated blood cells

The present invention is drawn to a hematology control made from particles a particle having a biopolymer attached to a surface of the particle. The particle simulates a component of a blood sample, such as a reticulocyte or nucleated red blood cell component of a blood cell sample in a flow cytometer or hematology analysis instrument. The present invention is further drawn to methods of making and using the hematology control.

Hematology controls for reticulocytes and nucleated red blood cells

A hematology reference control containing an immature granulocyte component and the method of making are disclosed. The reference control includes an immature granulocyte component made of processed blood cells for simulating human immature granulocytes, and a suspension medium suitable for delivering the component to a blood analyzer for measurement of immature granulocytes. The immature granulocyte component can be made of processed avian, reptile or fish red blood cells, or human immature granulocytes produced in vitro. The reference control further includes a white blood cell component which includes white blood cell sub-components for simulating white blood subpopulations. Further disclosed is the method of using the reference control for measurement of immature granulocytes on a blood analyzer.

Hematology reference control containing an immature granulocyte component

An improved blood suspension media for hematological compositions, having particular utility with a red blood cell component for devices using electronic and optical means for blood determinations, and processes for using the suspension media. The suspension media finds particular utility in providing the hematology control product with a stable and consistent MCV and RDW for an extended product shelf life.

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Fort Lauderdale, FL, United States of America

Theodore J Gerula