Koninklijke Philips Corporation N.V.

Siemens Healthineers Nederland B.v.

Concateno UK Limited

Koninklijke Philips Electronics N.v.

Cozart Bioscience Limited

Thea Van Der Wijk

Albert Hendrik Jan Immink

Menno Willem Jose Prins

Coen Adrianus Verschuren

Alexander Marc Van Der Lee

Johannes Joseph Hubertina Barbara Schleipen

Dominique Maria Bruls

Femke Karina De Theije

Eduard Gerard Marie Pelssers

Marius Iosif Boamfa

Richard Joseph Marinus Schroeders

Menno W J Prins

Remco Den Dulk

Gordon Thomas Jowett

Wilhelmina Maria Hardeman

Joost Hubert Maas

Dirkjan Bernhard Van Dam

Maatje Koets

Albert H J Immink

Sandra Marlin

Jessica Amadio

Femke Karina De Theije E/v Wijgergangs

Michel J M Bruyninckx

Eduard G M Pelssers

Menno WJ Prins

Richard JM Schroeders

Gordon T Jowett

André Albert Hendrik Jan Immink

The present invention relates to methods and (bio)sensor systems. Herein, magnetic fields are applied in order to transport magnetic particles laterally over a sensor surface with analyte specific probes. The methods of the invention allow the specific binding of magnetic particles to the sensor surface, while aspecific and unbound particles are removed.

Rapid magnetic biosensor

The invention relates to a method for the detection of target components that comprise label particles, for example magnetic particles (). The method includes (a) collecting the target components at a binding surface () of a carrier (); (b) directing an input light beam (L, L, L) into the carrier such that it is totally internally reflected in an investigation region () at the binding surface (); and (c) determining the amount of light of an output light beam (L, L, L) that comprises at least some of the totally internally reflected light. Evanescent light generated during the total internal reflection is affected (absorbed, scattered) by target components and/or label particles () at the binding surface () and will therefore be missing in the output light beam (L). This can be used to determine the amount of target components at the binding surface () from the amount of light in the output light beam (L, L, L). A magnetic field generator () is optionally used to generate a magnetic field (B) at the binding surface () by which magnetic label particles () can be manipulated, for example attracted or repelled.

Method of detecting label particles

The invention relates to a carrier with a binding surface at which target components that comprise label particles, for example magnetic particles, can collect and optionally bind to specific capture elements. An input light beam (L) is transmitted into the carrier and totally internally reflected at the binding surface. The amount of light in the output light beam (L) and optionally also of fluorescence light emitted by target components at the binding surface is then detected by a light detector. Evanescent light generated during the total internal reflection is affected (absorbed, scattered) by target components and/or label particles at the binding surface and will therefore be missing in the output light beam (L). This can be used to determine the amount of target components at the binding surface from the amount of light in the output light beam (L, L, L). A magnetic field generator is optionally used to generate a magnetic field (B) at the binding surface by which magnetic label particles can be manipulated, for example attracted or repelled.

Carrier for detecting label particles

The application relates to a method for detecting a target in a sample suspected of containing the target. The method comprises contacting the sample and a first binding molecule attached to a magnetic particle with a second binding molecule attached to a solid support. The first binding molecule is capable of binding to the second binding molecule, and the target is capable of interfering with this binding. Magnetic force is applied to bring the magnetic particle into close proximity with the solid support. The number of magnetic particles bound to the solid support is detected.

Detection of target molecules in a sample by using a magnetic field

The present invention discloses microfluidic devices with a valve-like structure (3), through which magnetic particles can be transported with minimal transport of fluids. This allows sequential processing of the magnetic particles.

Multi-compartment device with magnetic particles

A system and method wherein components of a reagent such as labeled antibodies are separated from a biologically active sensor surface by depositing the reagent on a carrier surface distinct from a sensor surface in a detection region. The present device provides a short, well-defined and controlled, pre-incubation time between the particles of interest in the sample fluid and the reagent, thereby increasing the reproducibility by providing all components in one detection region such as a detection chamber.

Methods and systems for detecting

A microelectronic sensor device for the detection of target components with label or magnetic particles includes a carrier with a binding surface at which target components can collect and optionally bind to specific capture elements. An input light beam is transmitted into the carrier and totally internally reflected at the binding surface. The amount of light in the output light beam is detected by a light detector. Evanescent light generated during the total internal reflection is affected by target components and/or label particles at the binding surface and will be missing in the output light beam. This is used to determine the amount of target components at the binding surface from the amount of light in the output light beam. A magnetic field generator is optionally used to generate a magnetic field at the binding surface by which magnetic label particles can be manipulated, such as attracted or repelled.

Microelectronic sensor device for detecting label particles

A detection system () and a sensor chip () for detecting target molecules, and thus corresponding analytes in a sample is described. Typically the detection system () includes a sensor chip (). The sensor chip () comprises on its detection surface () a dissolvable reagent layer (). When the dissolvable reagent layer () is in contact with sample fluid, free reagent is generated, assisting in the interaction between a label and target molecules, thus allowing for label based detection. The sample thereby is exposed to mobile reagents in a burst. The reagent layer may contain an enzyme allowing enzymatic assays.

Fast biosensor with reagent layer

Growing community of inventors

Bunnik, Netherlands

Thea Van Der Wijk