Dana-Farber-Cancer Institute Inc.

The Brigham and Women's Hospital, Inc.

Thanh Barbie

David Barbie

Kwok-kin Wong

Amir Aref

Russell W Jenkins

Shunsuke Kitajima

Israel Cañadas

Provided herein are Stimulator of Interferon Genes (STING) and Stimulated 3 Prime Antisense Retroviral Coding Sequences (SPARCS) genes as biomarkers for determining an effective therapy for treating cancer. Further provided are methods for treating cancer using said biomarkers.

STING levels as a biomarker for cancer immunotherapy

Provided herein are methods for culturing patient-derived tumor cell spheroids in a three-dimensional microfluidic device. The method comprises mincing primary tumor sample in a medium supplemented with serum; treating the minced primary tumor sample with a composition comprising an enzyme; collecting tumor spheroids having a diameter of 10 &#x3bc;m to 500 &#x3bc;m from the enzyme treated sample; suspending the tumor spheroids in biocompatible gel; and culturing the tumor spheroids in a three dimensional microfluidic device. Methods for identifying an agent for treating cancer and microfluidic devices that allow for the simultaneous exposure of the cultured patient-derived primary tumor cell spheroids to a treatment of choice and to control treatment are also provided.

Microfluidic cell culture of patient-derived tumor cell spheroids

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Andover, MA, United States of America

Thanh Barbie