Her Majesty the Queen in Right of Canada, As Represented by the Minister of Agriculture and Agri-food

Th&eacute;r&egrave;se Ouellet

Brian Miki

John A Simmonds

Jiro Hattori

Teresa Martin-Heller

Keqiang Wu

Kamal Malik

Lining Tian

Jas Singh

Elizabeth Foster

Peijun Zhang

Daniel C Brown

Raymond Tropiano

David A Theilmann

Titus Tao

The present invention is directed to translational regulatory elements that mediate the amount of protein produced within a host capable of expressing a construct comprising one or more translational regulatory elements in operative association with a gene of interest. These translational regulatory elements were derived from T1275 (tCUP) and exhibit a high degree of similarity with members of the RENT family of repetitive elements. Translational regulatory elements are disclosed that either increase or decrease he amount of protein produced within the host organism. These translational elements are operative in a wide range of hosts including plant, animals, yeast, fungi and bacteria. Analogs, derivatives and fragments of these translational elements are also disclosed.

Translational regulatory elements

The invention can be summarized as follows. Several silk-specific genes were isolated from corn silk. The specificity of their expression was determined by Northern analysis. A regulatory region was obtained from the silk gene, C3, and found to direct silk, and pistil, expression in transient assays and transgenic plants, in both monocot and dicot plants. The regulatory region of a silk gene may be used to drive the expression of a gene of interest within pistil or silk-tissues for a range of utilities including pathogen resistance or female sterility.

Corn silk gene and regulatory region

Growing community of inventors

Nepean, Canada

Thérèse Ouellet

Growing community of inventors

Nepean, Canada

Th&eacute;r&egrave;se Ouellet

Thérèse Ouellet