Empresa Brasileira De Pesquisa Agropecuaria-embrapa

The University of Melbourne

Thales Lima Rocha

Maria Cristina Mattar Da Silva

Maria Fátima Grossi De Sá

Ute Roessner

Maria Fatima Grossi De Sa

Tony Bacic

Raphael Garcia De Sousa Evaristo

Djair Dos Santos De Lima E Souza

Vera Lúcia Perussi Polez

Gustavo Ramos De Oliveira

Mariana Torquato Quezado De Magalhaes

Rafael Perseguini Del Sarto

The present invention relates to the field of insect pests control, using methods and compositions which comprise of alpha-amylase analogous mutant inhibitors (&#x3b1;AIs). More specifically, the invention provides new &#x3b1;AIs analogous mutant molecules for controlling insect pests, in particular boll weevils (), partially or totally presenting reduction of the amylolytic activity of the digestive enzymes in the intestinal lumen of the insect. Other aspects of the invention include gene constructs containing the nucleic acid molecules that code for the alpha-amylase inhibitors, heterologous expression methods of the new molecules in the active form, and the use of these molecules for controlling insect pests. The invention also provides analogous synthetic genes optimized for their transformation and expression in plants.

Alpha amylase mutant inhibitors isolated from phaseolus vulgaris with properties of controlling insect pests, compositions containing such mutants, and method of using thereof

The present application relates generically to compositions used for controlling agricultural pests and, in a particular, to compositions usable for combating nematodes. The application presents a feasible alternative to the use of nematotoxic compounds that are harmful to the environment and that may damage human health. This alternative includes a nematotoxic composition for controlling nematodes that may be used on plants, parts of plants and on the soil. The respective technology results from unexpected results achieved from experiments carried out with extracts of the plant, resulting in an unheard-of nematotoxic composition comprising a specific combination of substances that are naturally found in the extract and that act synergistically.

Nematotoxic composition of synergistic effect, use of a nematotoxic composition of synergistic effect

The present invention belongs to an insecticide compound derived from a strain ofand refers to the field of controlling plant pests, particularly to the control of the boll weevil&#x2014;. More specifically, the object of the invention refers to a new gene for the new delta-endotoxin designated Cry8Ha and to the cloning and expression of the gene that encodes for the protein Cry8Ha in. There is provided the nucleotide sequence and encoding protein of the new delta-endotoxin, recombinant vectors and host cells. There is also provided processes and means for recombinant production and the use of the new delta-endotoxin for application in the control of the boll weevil. Additionally, the invention also provides an optimized synthetic gene for expression in cotton plants. Using the gene described herein, it is possible to transform plants based on techniques known by specialists in the art, for the expression of the endotoxin active against the boll weevil.

Genetic construct expressing insecticidal toxin and the method of using thereof

The present invention relates generically to compositions used for controlling agricultural pests and, in a particular embodiment, to compositions usable for combating nematodes. The invention presents a feasible alternative to the use of nematotoxic compounds that are harmful to the environment and that may damage human health. This alternative is embodied through a nematotoxic composition for controlling nematodes and that may be used on plants, parts of plants and on the soil. The respective technology results from unexpected results achieved from experiments carried out with extracts of the plant, resulting in an unheard-of nematotoxic composition comprising a specific combination of substances that are naturally found in the extract and that act synergistically.

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Brasilia, Brazil

Thales Lima Rocha