Mogen International

Gist-brocades N.v.

Gist-brocades B.v.

Mogen International Nv

Syngenta Mogen B.v.

Mogen International and Gist-brocades N.v.

Teunis Cornelis Verwoerd

Andreas Hoekema

Jan Pen

Albert J Van Ooijen

Krijn Rietveld

Peter Christiaan Sijmons

Peter Christian Sijmons

Wilhemus J Quax

Wilhelmus J Quax

Oscar Johannes Maria Goddijn

Albert Johannes Joseph Van Ooyen

Eline Voogd

Krun Rietveld

Ronny Wilhelmus Hermanus Henrika Krutwagen

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Production of enzymes in seeds and their use

The invention provides a process for producing trehalose in plant cells capable of producing trehalase by growing plant cells having the genetic information required for the production of trehalose and trehalase, or cultivating a plant or a part thereof comprising such plant cells, characterized in that said plant cells are grown, or said plant or a part thereof, is cultivated in the presence of a trehalase inhibitor.

Enhanced accumulation of trehalose in plants

The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.

Expression of phytase in plants

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Growing community of inventors

Leiden, Netherlands

Teunis Cornelis Verwoerd