Japan Science and Technology Agency

Japan Science and Technology Corporation

Tetsuya Yomo

Kenji Yasuda

Satoshi Fujii

Hajime Watanabe

Yuichi Wakamoto

Tomoaki Matsuura

Haruka Soga

Kunihiko Kaneko

Ippei Inoue

The objective of the present invention is to improve the efficiency of screening/selection of a membrane protein in molecular evolutionary engineering (for example, an enzyme evolutionary method). The above-described objective is achieved by providing a unilamellar liposome comprising:

In vitro membrane protein molecular evolutionary engineering technique

An apparatus is provided with a cell culture container having a cell culture region made of a hole formed on a substrate, a semi-permeable membrane covering a top plane of the cell culture region and a culture medium replacement part provided over the semi-permeable membrane, a mechanism for supplying a cell culture medium into the cell culture container, and a microscopic optical mechanism for enabling long-term observation of the cell within the cell culture region. This apparatus makes it possible to culture a cell group originating from a particular single cell, to perform culture and observation while identifying cells to be subjected to interaction during the process of culturing cells, and observe a difference in variation between the particular cell and other cells. There is also provided a mechanism which makes it possible to collect only a cell assuming a particular state and perform analysis or biochemical measurement of a gene of the cell, an expressed mRNA and the like.

Apparatus for microscopic observation of long-term culture of single cell

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Osaka, Japan

Tetsuya Yomo