Kikkoman Corporation

Noda Institute for Scientific Research

Kokkoman Corporation

Tetsuya Oguma

Nobuyuki Yamaji

Shoichi Tokutake

Kiyoshi Mizusawa

Koichiro Tobe

Tatsuo Horiuchi

Mamoru Kikuchi

Makoto Saito

Yasuji Koyama

Shigehiro Kataoka

Takeharu Nakahara

Satoshi Fukuda

Jun Yamakoshi

Kotaro Ito

Katsumi Yuasa

Hitomi Aota

Norikazu Fujii

Yasuhiko Imai

Satoshi Kitao

Kohsuke Matsumoto

Hirokazu Koyama

Kahee Fujita

A low common salt soy sauce, which has good flavor, significantly suppresses elevation of blood pressure, prevents cardiac hypertrophy, and is also available as special nutritious food, is obtained. 1.0% to 10.0% by weight of potassium chloride and 0.1% to 5.0% by weight of &#x3b3;-aminobutyric acid are added to a reduced common salt soy sauce, so as to obtain a low common salt soy sauce of issue. Otherwise, a KCl-containing low common slat soy sauce is obtained by: (1) a common production method of soy sauce, in which a mixed solution consisting of KCl and common salt is used as mother water; (2) a method of subjecting a soy sauce obtained using saline solution as mother water to electrodialysis, a membrane treatment, or the like, so as to eliminate common salt from the above soy sauce, and then adding KCl thereto; or other methods. Thereafter, &#x3b3;-aminobutyric acid is added to the above KCl-containing low common salt soy sauce, so as to obtain a low common salt soy sauce comprising 0% to 10% by weight of common salt, 1.0% to 10.0% by weight of potassium chloride, and 0.1% to 5.0% by weight of &#x3b3;-aminobutyric acid.

Low-salt soy sauce

There are disclosed a protein having an amino acid sequence shown in SEQ ID NO:2, or a protein having a glutaminase activity in which one or more amino acids is/are deleted from, substituted by, inserted to or added to the amino acid sequence of the above protein; a gene containing DNA encoding the above protein or a gene which hybridizes with the a complementary sequence of DNA of the above gene under a stringent condition and encodes a protein having a glutaminase activity; a recombinant DNA containing the above gene; a transformant or a transductant containing the above recombinant DNA; and a process for producing glutaminase which comprises culturing the above transformant or the above transductant and collecting glutaminase from a culture medium.

Protein having glutaminase activity and gene encoding the same

A cyclic oligosaccharide or salts thereof comprising glucoses bound cyclically via .alpha.-1,6-linkages and having at least one S-oxo acid group bound, and ana gent for preventing or treating retroviral diseases which comprises said cyclic oligosaccharide or salts thereof as an active ingredient. The novel sulfated cyclic isomaltoligosaccharide inhibits infection of host cells with various retroviruses such as AIDS virus etc. so effectively that it has a significant preventive and therapeutic effect on diseases caused by retroviruses. Accordingly, said oligosaccharide can be utilized as an agent for preventing or treating retroviral diseases in pharmaceutical industry.

Cyclic oligosaccharide, and an agent containing the same for preventing

The present invention relates to a novel cycloisomaltooligosaccharide selected from the group consisting of novel cycloisomaltoheptaose having a cyclic structure composed of 7 glucose residues in .alpha.-1,6 linkage, novel cycloisomaltooctaose having a cyclic structure composed of 8 glucose residues in .alpha.-1,6 linkage and novel cycloisomaltononaose having a cyclic structure composed of 9 glucose residues in .alpha.-1,6 linkage, novel cycloisomaltooligosaccharide synthase forming said oligosaccharides from dextran, and a process for producing said oligosaccharides by use of said enzyme or a microorganism capable of producing said enzyme.

Enzyme for producing novel cyclooisomaltooligosaccharides

Cycloisomaltooligosaccharides

The present invention relates to the preparation of a cylodextrinase having specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular weight by culturing a specified microorganism belonging to the genus Bacillus, and also provides a process for preparing maltooligosaccharides by using the present enzyme on cylodextrins.

Preparation and use of a cyclodextrinase for preparing

A process for producing ellagic acid comprising reacting a tannin having gallic acid residues attached as constituents with an oxidizing agent at pH 7 or higher.

Process for producing ellagic acid

The present invention relates to a novel cyclodextrinase having the specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular, weight. This novel enzyme can be produced by culturing a specified microorganism belonging to the genus Bacillus. The invention also provides a process for preparing maltooligosaccharides by acting the present enzyme to cyclodextrins.

Purified cyclodextrinase

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Noda, Japan

Tetsuya Oguma