Ajinomoto Co., Ltd.

Tetsuya Miwa

Nami Nakamura

Keiichi Yokoyama

Noriki Nio

Katsuya Seguro

Hiroaki Kisaka

Minao Asano

Shuichi Yanagisawa

Ai Akiyama

Misako Kawai

Yaqin Lang

Daiki Ninomiya

Ryuji Sugiyama

Susumu Yazawa

The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modified plant capable of producing capsinoids, which shows a decreased expression or activity of an enzyme that catalyzes an amino group conversion reaction from vanillin to vanillylamine as compared to wild strains, and the like. As a method of suppressing expression or activity of an enzyme, an introduction of DNA encoding an antisense RNA, iRNA, ribozyme or dominant-negative mutant for the target gene, a destruction of the gene by a knockout method, mutagen treatment or transposon insertion, an introduction of a gene encoding an antibody against the enzyme and the like can be mentioned.

Genetically modified plant capable of biosynthesizing capsinoid

The object of the present invention is to provide a transformed plant having an enhanced amino acid content, a progeny of the plant, seeds of the plant and a method for producing the plant. The present invention provides a transformed plant having a gene encoding DNA binding protein of Dof family and also having a free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions. The present invention also provides a method of producing a transformed plant having the free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions by introducing a gene encoding DNA binding protein of Dof family into the plant to express the gene in the plant body.

Plants having an enhanced amino acid content, plants having an enhanced nitrogen content, plants tolerant to nitrogen deficiency, and methods for producing them

The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

Potatoes having an increased yield of starch per plant body and method for producing the same

Disclosed are a protein having a transglutaminase activity, which comprises a sequence ranging from serine residue at the second position to proline residue at the 331st position in an amino acid sequence represented by SEQ ID No. 1 wherein the N-terminal amino acid of the protein corresponds to serine residue at the second position of SEQ ID No. 1, a DNA encoding the protein, a transformant having the DNA, and a process for producing a protein having a transglutaminase activity, which comprises the steps of culturing the transformant in a medium. The protein can be produced in a large amount with the transformant using a host such as

Process for producing microbial transglutaminase

DNA molecule encoding new aminopeptidase, and method of producing the aminopeptidase

An aminopeptidase is provided which efficiently decomposes a low-molecular-weight peptide containing glutamic acid or aspartic acid in its sequence. A method of hydrolyzing a peptide or protein by use of the aminopeptidase is also provided. Aminopeptidase GX is derived from germinated soybean cotyledons and releases glutamic acid or aspartic acid from a peptide or protein containing glutamic acid or aspartic acid at the N-terminal end and is used to hydrolyse peptides or proteins.

Aminopeptidase GX, and a method of hydrolyzing a protein with the same

Disclosed are a protein having a transglutaminase activity, which comprises a sequence ranging from serine residue at the second position to proline residue at the 331st position in an amino acid sequence represented by SEQ ID No. 1 wherein the N-terminal amino acid of the protein corresponds to serine residue at the second position of SEQ ID No. 1, a DNA encoding the protein, a transformant having the DNA, and a process for producing a protein having a transglutaminase activity, which comprises the steps of culturing the transformant in a medium. The protein can be produced in a large amount with the transformant using a host such as E. coli.

Growing community of inventors

Kawasaki, Japan

Tetsuya Miwa