Hiroshima University

Astellas Pharma Gmbh

National Center for Global Health and Medicine

Riken Corporation

Kyushu University

Repertoire Genesis Incorporation

Reperptoire Genesis Incorpration

Tetsushi Sakuma

Takashi Yamamoto

Hiroshi Ochiai

Shinya Matsuura

Tatsuo Miyamoto

Hideki Endo

Yukihito Ishizaka

Akira Ishiguro

Ryuji Suzuki

Yasuko Honjo

Tatsuo Ichinohe

Takahiko Miyama

Takakazu Kawase

Tomoki Takashina

Yusuke Yagi

Takahiro Nakamura

Keiji Numata

Hiroshi Ezura

Taku Demura

Takeshi Yoshizumi

Yutaka Kodama

Yasuyuki Okawa

Yoko Horii

Masakazu Saito

Kenji Miura

Yuto Sakane

Kenichi Suzuki

The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

Polypeptide containing DNA-binding domain

The present invention provides an artificial nucleic acid-cleaving enzyme comprising: a nuclease domain which is a polypeptide containing an amino acid sequence set forth at positions 391 to 585 of SEQ ID NO: 1 or positions 389 to 579 of SEQ ID NO: 3, or a mutant polypeptide thereof; and a nucleic acid-binding domain.

Nuclease domain and use thereof

An object of the present invention is to provide a cell penetrating peptide having a penetrating ability into cells. The present inventors provided a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 1, a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 2 and a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 3; and a complex comprising any one of the cell penetrating peptide and a functional molecule.

Cell penetrating peptide

The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.

Full replacement technique for T cell receptor using platinum TALEN

The present disclosure provides a technique for producing regulatory T cells specific to a desired antigen. Regulatory T cells specific to an antigen are produced by a method that includes: a step for identifying a T cell receptor (TCR) clone present in an effector T cell population specific to an antigen in an effector T cell donor; and a step for introducing all or part of the nucleic acid sequence of the TCR&#x3b1; gene and all or part of the nucleic acid sequence of the TCR&#x3b2; gene included in the clone into a regulatory T cell, said step comprising introducing the TCR&#x3b1; and the TCR&#x3b2; so as to be expressed in pairs.

Technique for creating antigen-specific regulatory T cells (Treg) in which effector T cell (Teff) antigen receptors are used

The present application provides a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 1, a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 2 and a cell penetrating peptide consisting of the amino acid sequence of SEQ ID NO: 3; and a complex comprising any one of the cell penetrating peptide and a functional molecule.

It is intended to provide a fusion polypeptide that regulates the transcription of a target gene. The present inventors have provided a fusion polypeptide comprising: a cell-penetrating peptide; a DNA-binding polypeptide; and a transcriptional regulator.

Transcriptional regulatory fusion polypeptide

It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.

Method for introducing protein into plant cell

The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an &#x3b1;-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an &#x3b1;-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. 'ii' (&#x2212;2) A. A. contained in L of the formula 1, the aforementioned object could be achieved.(Helix A)-X-(Helix B)-L&#x2003;&#x2003;(Formula 1)

DNA-binding protein using PPR motif, and use thereof

Growing community of inventors

Higashihiroshima, Japan

Tetsushi Sakuma