Kyowa Hakko Bio Co., Ltd.

Tetsuro Ujihara

Satoshi Mitsuhashi

Yasuharu Satoh

Shohei Hayashi

Tohru Dairi

Kazuhiko Tabata

Makoto Yagasaki

Tetsuya Abe

Yasushi Ogasawara

Megumi Nagano

Mai Naka

An object of the present invention is to provide a microorganism that efficiently produces a PUFA and a method for producing a PUFA using the microorganism. The present invention relates to a microorganism capable of producing a polyunsaturated fatty acid (PUFA), in which a gene encoding an exogenous polyketide synthase dehydratase (PS-DH) domain having a higher activity against 3-hydroxyhexanoyl acyl carrier protein (3-hydroxyhexanoyl ACP) than an endogenous FabA-like β-hydroxyacyl-ACP dehydratase (FabA-DH) domain has been introduced into a microorganism having a PUFA metabolic pathway, and the like.

Microorganism producing polyunsaturated fatty acid and method for producing polyunsaturated fatty acid

An object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in which at least one of the amino acid residues at positions 6, 65, 230, 231, and 275 in the amino acid sequence represented by SEQ ID NO: 2 has been substituted with another amino acid residue (mutated OrfB), and is capable of producing eicosapentaenoic acid (EPA), and the like.

Microorganism producing eicosapentaenoic acid and method for producing eicosapentaenoic acid

According to the present invention, there can be provided a process for producing 7-dehydrocholesterol (7DHC), comprising culturing, in a medium, a 7DHC-producingmicroorganism in which sterol 24-C-methyltransferase activity is reduced or lost as compared to a parent strain, allowing 7DHC to be produced and accumulated in the culture, and collecting the 7DHC from the culture; and a process for producing vitamin D3, comprising irradiating, with ultraviolet light, 7-dehydrocholesterol produced by the production process.

Process for producing 7-dehydrocholesterol and vitamin D3

According to the present invention, there can be provided A process for producing 7-dehydrocholesterol (hereinafter, '7DHC'), comprising culturing, in a medium, a 7DHC-producingmicroorganism in which the 7DHC reducing activity is reduced or lost as compared to a parent strain through deletion, substitution, or addition of at least one base in a gene which is present in the chromosomal DNA of the parent strain and encodes a protein having 7DHC reducing activity, and the microorganism produces 7DHC, allowing 7DHC to be produced and accumulated in the culture, and collecting the 7DHC from the culture; and a process for producing vitamin D3, comprising irradiating, with ultraviolet light, the 7DHC produced by the production process.

According to the present invention, a microorganism belonging to the genushaving an 18S rRNA gene consisting of the base sequence represented by any of SEQ ID NOS: 1 to 5;sp. OH4 strain; a microorganism which is a mutant obtained from the above-mentioned microorganism as a parent strain and has a higher ability to produce DHA than the parent strain; orsp. LTR23 strain is provided. Also, a method for producing a DHA-containing composition, DHA and DHA alkyl ester by a fermentation process using the above-mentioned microorganisms is provided.

Microorganism producing docosahexaenoic acid and utilization thereof

A target substance can be efficiently produced by culturing, in a medium, a coryneform bacterium in which the activity of a PTS protein relating to fructose uptake is reduced or lost as compared with a parent strain and the bacterium can produce the target substance, allowing the target substance to form and accumulate in a culture; and collecting the target substance from the culture.

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Tokyo, Japan

Tetsuro Ujihara