Matsushita Electric Industrial Co., Ltd.

Panasonic Corporation

Tetsuo Yukimasa

Hiroaki Oka

Hidenobu Yaku

Masaya Nakatani

Yukari Hataoka

Ryuta Ogawa

Hirokazu Sugihara

Nobuhiko Ozaki

Maki Yotsuhashi

Tohru Nakagawa

Katsuyuki Tsuji

Kimi Fukahi

Maki Katagiri

Yukifumi Yoshimoto

A nucleic acid amplification reaction vessel has a substrate, a cavity formed in the substrate, a cover plate for sealing the cavity, and a sample-injection inlet formed in the cover plate. The cavity includes a columnar structure connected to the cover plate or the substrate. The nucleic acid amplification reaction vessel allows fast temperature increase or decrease of sample liquid.

Amplification reaction vessel, and method of manufacturing the same

The present invention provides a kit employed for polymerase chain reaction. The kit has a cavity and a flow channel on a substrate. The flow channel is separated from the cavity by at least a barrier formed along the cavity. Such a structure allows the cavity to be filled with a sample solution even in minute quantities. At the same time, the structure can provide a sample solution with a rapid temperature-control. The structure can therefore contribute to accelerated polymerase chain reaction.

Polymerase chain reaction kit and method of manufacturing the same

A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided. A sensor of pyrophosphate which is characterized by including: an insulative substrate; an electrode system that is formed thereon and has a measurement electrodeand a counter electrode; and a plurality of reaction reagent layers that are provided on the substrateand include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layerincluding the enzyme being separated from reaction reagent layerincluding the buffer component, and reaction reagent layerincluding glyceraldehyde-3-phosphate being separated from the reaction reagent layerincluding the buffer component.

Sensor of pyrophosphate and SNP typing sensor using the same

A fluid chip has a liquid supportfor supporting a liquid, a fluid passageformed by being surrounded by the liquid supportand through which the liquidmoves, and a plurality of magnetic particlesdisposed in the fluid passage. The magnetic particlesare aggregated by a permanent magnetso as to block the fluid passageand thus cut off flow of the liquid. The magnetic particlesare dispersed along a part of a wall of the fluid passageso as to open the fluid passageand thus allow the liquidto flow.

Fluid chip, fluid movement control method using the same, and chemical reaction apparatus

A liquid delivery apparatushas a rotary substrate. The substrateformed with a fluid passageA for communicating a supply chamberA and target chamberA. An inlet end portionat which the fluid passageA is connected to the chamberA extends in a clockwise direction Rof rotational directions of the substrate. The inlet end portionholds a liquidin the chamberA by a capillary force. The rotation drive unitrotates the substrateso that an inertial force exceeding the capillary force and directed to the clockwise direction Racts on the liquidin the inlet end portion. Delivery behavior control of microfluid with a high degree of flexibility can be achieved.

Liquid delivery apparatus and liquid delivery method

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be T or G; the third base from the 3&#x2032; end to be any one of T or G; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

Discrimination method of target base in DNA, and allele specific primer used in the method of the same

Polymerase chain reaction container and process for producing the same

The present invention provides a blood processing apparatus that can introduce a metered quantity of a blood sample into a chamber and does not require an opening linked to the chamber to be closed after the introduction of the blood sample. An introducing chamber, an injection port, and an air port are formed in a rotatable platform of a blood processing apparatus. The injection port links the introducing chamber with the outside of the rotatable platform. A blood sample is introduced from the injection port into the introducing chamber. A blood coagulant is held on the port wall surface of the air port and/or around a portion of the surface of the rotatable platform where the air port is open.

Blood processing apparatus and blood introducing method

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be T or G; the third base from the 3&#x2032; end to be any one of A, T or C; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is G, while a base adjacent with one base spaced apart is T. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be T or G; the third base from the 3&#x2032; end to be G or C; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is A, while a base adjacent with one base spaced apart is G. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be C; the third base from the 3&#x2032; end to be any one of A, T or G; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is A, while a base adjacent with one base spaced apart is T. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be C; the third base from the 3&#x2032; end to be any one of T, C or G; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is C, while a base adjacent with one base spaced apart is C. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second and the third bases from the 3&#x2032; end to be 5&#x2032;-AT-3&#x2032;, 5&#x2032;-TA-3&#x2032;, 5&#x2032;-TT-3&#x2032; or 5&#x2032;-CT-3&#x2032;; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is C, while a base adjacent with one base spaced apart is T. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be T; the third base from the 3&#x2032; end to be any one of T, G or C; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

Growing community of inventors

Nara, Japan

Tetsuo Yukimasa