Kyowa Hakko Kogyo Co., Ltd.

Abbott Laboratories Corporation

Other

Juridical Foundation, Japanese Foundation for Cancer Research

Tetsuo Oka

Ryoichi Katsumata

Akira Furuya

Akio Ozaki

Tamio Mizukami

Toru Mizukami

Isao Kawamoto

Takashi Nara

Tatsunari Nishi

Seiga Itoh

Ryo Okachi

Tomoyasu Sato

Masaji Kasai

Kunikatsu Shirahata

Tadashi Matsumoto

Tadatsugu Taniguchi

Tatsuya Tamaoki

Fusao Tomita

Haruo Sugano

Haruhiko Yokoi

Seiga Ito

Nobuo Nakamura

Shuji Okubo

Motoko Kageyama

Morimasa Yagisawa

Disclosed is a process for expressing a gene and producing a metabolic product formed by the gene by culturing a transformant microorganism carrying a recombinant DNA constructed of a DNA fragment having at least one gene to be expressed and a vector DNA, at least one of which is foreign to the host microorganism.

Amino acid synthesis in corynebacteria using E. coli genes

A process for producing L-lysine is disclosed in which a recombinant vector, a DNA fragment of which contains a gene involved in the synthesis of dihydropicolinic acid synthetase, is used to transform a microorganism of the genus Corynebacterium or Brevibacterium. The transformant is then cultured in a medium which supports the accumulation of L-lysine. Subsequently the L-lysine is recovered from the culture broth.

Process for producing L-lysine

Disclosed is a process for producing histidine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of histidine and a vector DNA, culturing the transformant in a nutrient medium, accumulating histidine in the culture medium and recovering histidine therefrom.

Process for producing histidine

The invention relates to a bacterial process for producing phenylalanine. The process utilizes a Corynebacterium or Brevibacterium host which is transformed with a recombinant DNA. The recombinant DNA harbors a DNA containing a gene which encodes chorismate mutase or prephenate dehydratase. The transformed microorganism is then cultured in order to accumulate phenylalanine in the culture medium and the phenylalanine is recovered therefrom.

Process for producing phenylananine

Disclosed is a process for producing tryptophan by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of tryptophan and a vector DNA, culturing the transformant in a nutrient medium, accumulating tryptophan in the culture medium and recovering tryptophan therefrom.

Process for producing tryptophan

Disclosed is a process for producing L-arginine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of L-arginine and a vector DNA, culturing the transformant in a nutrient medium, accumulating L-arginine in the culture medium and recovering L-arginine therefrom.

Process for producing L-arginine

Process for producing peptides

Disclosed are recombinant plasmid vectors constructed of a plasmid autonomously replicable in cells of microorganisms belonging to the genus Corynebacterium or Brevibacterium and a DNA fragment containing a gene expressible by said microorganisms. The recombinant vector plasmids are autonomously replicable in glutamic acid--producing microorganisms and are useful to clone desired DNA fragments in such microorganisms.

Novel vector plasmids

Recombinant vector plasmids containing a DNA fragment coding for human interferon-.beta. inserted downstream from a tryptophan promoter are useful for transformation of microorganisms such as Escherichia coli which transformants produce human interferon-.beta..

Recombinant plasmid containing human interferon-beta gene

Disclosed is a method for transformation of microorganisms belonging to the genera Corynebacterium and Brevibacterium whereby a foreign DNA may be introduced into a host cell and autonomously replicated.

Method for transforming microorganisms

A novel lysozyme-sensitive microorganism belonging to the genus Corynebacterium or Brevibacterium and having a sensitivity to lysozyme at a concentration of less than 25 .mu.g/ml is provided from selected mutants. This novel microorganism is especially suitable for use in recombinant DNA technology.

Novel lysozyme-sensitive microorganism

Disclosed are novel plasmids which can replicate autonomously in microorganisms belonging to the genus Corynebacterium or Brevibacterium and which carry a gene for resistance to streptomycin and/or spectinomycin. The plasmids are useful as a cloning vector.

Plasmids

Disclosed is a novel vector, plasmid pCG2, and a process of producing plasmid pCG2 from Corynebacterium glutamicum.

Plasmid pCG2

A new antibacterial compound, DC-11 is produced by fermentation of a microorganism belonging to the genus Micromonospora. The antibiotic is accumulated in the culture medium and is isolated therefrom.

Antibiotic DC-11 and process for production thereof

The antibacterial compounds 7-deazaadenosine and 7-deazinosine are produced by fermentation of a new microorganism of the genus Micromonospora. At least one of the active substances is isolated from the culture medium.

Growing community of inventors

Yokohama, Japan

Tetsuo Oka