Takara Bio Inc.

Takara Shuzo Co., Inc.

Tetsuki Tanaka

Ikunoshin Kato

Takashi Uemori

Shigekazu Hokazono

Jun Tomono

Susumu Tsunasawa

Masaru Miyagi

Yukiko Izu

Tetsuo Tanigawa

A polypeptide having an RNaseH activity fromand being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide.

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An isolated polypeptide having a thermostable ribonuclease H activity fromis highly useful in genetic engineering, as well as a gene encoding this polypeptide and a genetic engineering process for producing the polypeptide.

To provide an amino terminal protecting group-releasing enzyme characterized in that the enzyme possesses an activity for releasing a protecting group by acting on a peptide of which amino terminal is blocked by the protecting group, and exhibits the activity for two or more protecting groups, or a functional equivalent thereof; a DNA encoding the same; a method for producing the enzyme; a method for removing amino terminal protecting group including the step of subjecting to a reaction with the enzyme to release amino terminal protecting group; and a method for analyzing an amino acid sequence. The above enzyme is useful in the analysis of an amino acid sequence of peptides, particularly proteins and peptides, of which amino terminal is blocked by unknown protecting groups.

Amino-terminal deblocking enzyme

Growing community of inventors

Moriyama, Japan

Tetsuki Tanaka