Fujirebio Inc.

Fuji Photo Film Company, Limited

Other

Fujirebio Kabushiki Kaisha

Fujirejiro Inc.

Tetsuji Tanimoto

Masashi Ogawa

Kazuya Takahashi

Yoshihiro Ashihara

Toshikage Hiraoka

Yukio Sudo

Yoshihiko Makino

Masahisa Okada

Shigeki Kageyama

Isao Nishizono

Nobuyuki Ise

Tadashi Ninomiya

Katsuya Fujimura

Naofumi Hora

Takeya Hori

Toshihiro Mori

Hiroshi Shinoki

Masami Sugiyama

Yoshiaki Uchida

Yoshihiro Kurano

Tatsuki Matsuno

Aiko Tanaka

Kazushige Moriyama

Eiji Miyagawa

The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

Chemiluminescence enhancer

A fused antigen in which at least two surface antigens of are fused and an assay for anti- antibodies, using the above fused antigen.

Treponema pallidum fused antigen and assay for anti-treponema pallidum antibodies using the same fused antigen

An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.

Immunoassay element and process for immunoassay

A,fused antigen in which at least two surface antigens of,are fused and an assay for anti-,antibodies, using the above,fused antigen.

A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,

Dry-type analytical element for immunoassay

An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.

Immunoassay element and process and immunoassay

A method for measuring glycated hemoglobin is disclosed, which comprises contacting a sample with an anti-hemoglobin antibody being bound to a solid phase, adding thereto an anti-glycated hemoglobin antibody, and then measuring the anti-glycated hemoglobin antibody bound to the solid phase via glycated hemoglobin and the anti-hemoglobin antibody. A method for measuring glycated hemoglobin is further disclosed, which comprises mixing a sample with particles on which an anti-glycated hemoglobin antibody is bound to cause immunoagglutination reaction. A method for measuring glycated hemoglobin is furthermore disclosed, which comprises mixing a sample with a solution containing a monoclonal antibody specific for glycated hemoglobin, and then measuring the change in the turbidity of the resulting mixture. A method is disclosed for measuring glycated hemoglobin which comprises mixing a sample with unsensitized latex particles and an anti-glycated hemoglobin antibody, to cause immunoagglutination reaction. There is disclosed a method for measuring glycated hemoglobin which comprises mixing a sample with unsensitized latex particles and particles on which an anti-glycated hemoglobin antibody is bound, to cause immunoagglutination reaction. Still furthermore, an anti-glycated hemoglobin monoclonal antibody having a high specificity for human glycated hemoglobin is disclosed.

Anti-glycated hemoglobin monoclonal antibody and method for measuring

Growing community of inventors

Tokyo, Japan

Tetsuji Tanimoto