Toray Industries, Inc.

Tetsu Yonehara

Katsushige Yamada

Reiko Miyata

Kenji Sawai

Hideki Sawai

Takashi Mimitsuka

Satoshi Sakami

Masahiro Henmi

Daijiro Tsukamoto

Yohito Ito

Kyousuke Yotsumoto

Hiromi Tsutsui

Makoto Shirai

Masae Takeuchi

A method for producing highly pure p-toluic acid by a simple solid-liquid separation operation using as a raw material a substance inducible from a biomass resource and suitable for fermentation production by a microorganism is disclosed. The method for producing p-toluic acid includes: a dehydration reaction step of dehydrating Compound(s) (1)-(4), which are substances inducible from biomass resources and suitable for fermentation production by microorganisms; and a solid-liquid separation step of recovering a solid produced by this dehydration reaction by a simple solid-liquid separation operation.

P-toluic acid production method

The invention provides a method of producing a chemical product through continuous fermentation which includes filtering a culture of a microorganism or cultured cells with a separation membrane to recover a product from a filtrate and simultaneously retaining a nonfiltered fluid in, or refluxing it to, the culture, and adding fermentation materials to the culture, wherein a porous membrane having an average pore size of 0.01 &#x3bc;m or more to less than 1 &#x3bc;m is used as the separation membrane and the filtration is conducted with a transmembrane pressure difference in the range of 0.1 to 20 kPa. According to this method, the fermentation productivity of the chemical product can be largely elevated at high stability and a low cost.

Method of producing chemical product with continuous fermentation and filtering

Disclosed is a method for producing folic acid, comprising incubating yeast having the ability to produce folic acid of 0.3 mg or more or incubating bacteria having the ability to produce folic acid of 1 mg or more per liter of the culture, thereby accumulating folic acid in the culture.

Method for producing folic acid

A process for production of inositol comprising the steps of (1) culturing a microorganism capable of extracellularly secreting inositol and belonging to the genus Candida in a medium so as to extracellularly accumulate inositol in the medium; and optionally (2) recovering inositol from the culture. Preferably, the producer microorganism is resistant to an antibiotic such as cerulenine or D-cycloserine, or resistant to a glucose metabolism antagonist such as 2-deoxyglucose.

Process for production of inositol and microorganism used therefor

It is possible to produce and accumulate D-alanine selectively and to improve the amount of production and accumulation of D-alanine by cultivating a microorganism having both an ability to produce D-alanine and a resistance to D-cycloserine and belonging to the genus Brevibacterium.

Process for producing D-alanine

Method for producing pyruvic acid by fermentation

Growing community of inventors

Aichi, Japan

Tetsu Yonehara