Kaneka Corporation

National Institute of Advanced Industrial Science and Technology

Kobe University

Teruyuki Nishi

Kiyoshi Asai

Masayuki Machida

Hiroko Hagiwara

Akihiko Kondo

Tozo Nishiyama

Yasuyuki Nakamura

Jun Ishii

Yuji Okubo

Yoichiro Ito

Toru Watanabe

One or more embodiments of the present invention are to provide a new means of improving the productivity of a target protein. The present inventors have identified a novel protein consisting of the amino acid sequence set forth in SEQ ID NO: 2 through an exhaustive analysis of the nucleotide sequence of chromosomal DNA of a yeast belonging to the genus. Activation of a gene encoding the novel protein provides a cell having an improved productivity of a target protein.

Cell and method for producing target protein using same

A host cell includes an inactivated gene. The inactivated gene may be a gene including the nucleotide sequence of SEQ ID NO: 34, a gene including a nucleotide sequence of a nucleic acid hybridizing under a stringent condition to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO: 34, a gene comprising a nucleotide sequence having a sequence identity of 85% or more to the nucleotide sequence of SEQ ID NO: 34, or a gene encoding an amino acid sequence having a sequence identity of 85% or more to the amino acid sequence of SEQ ID NO: 33.

Host cell and method for producing target protein using same

A vector is provided that includes a nucleotide sequence selected from any one of (a) to (d). The selection of (a) to (d) includes: (a) the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21, (b) the nucleotide sequence complementary to the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21, where (a) and (b) hybridize under stringent conditions, (c) the nucleotide sequence having 90% or more sequence identity with the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21, and (d) the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21 in which a total of 1 to 50 nucleotides are substituted, deleted, or inserted.

Vector containing centromere DNA sequence and use thereof

This invention provides a novel means that allows efficient secretion and production of a target protein in a host cell. This invention concerns a novel polypeptide having an activity of improving the secretion productivity of a target protein, a gene comprising a nucleotide sequence encoding such polypeptide, a vector comprising such gene, a transformant obtained by transforming a host cell with such vector, and a method for producing a protein comprising a step of culturing such transformant and recovering a target protein from the culture product.

Polypeptide, and use thereof

Growing community of inventors

Hyogo, Japan

Teruyuki Nishi