Other

National Institute of Advanced Industrial Science and Technology

Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

Eiken Nagaku Kabushiki Kaisha

Teruyuki Nagamune

Hiroshi Ueda

Hajime Nishimura

Greg Winter

Paula A Schueler

Izumi Kumagai

Kouhei Tsumoto

Walter C Mahoney

Koichi Kato

Jun Miyake

Masato Miyake

Yoshiyuki Ohiro

Norio Shibata

Hideki Inaba

Susumu Tachikawa

The present invention provides a method for controlling the spatial arrangement of an energy donor and an energy acceptor in a reaction complex in order to carry out the measurement with good precision and high sensitivity, and a measurement system using the method. Two types of material having affinity for the subject material to be measured are respectively labeled with a combination of reporters that give rise to energy transfer; those that have been thus obtained by labeling these materials are each further labeled with materials that have weak affinity for each other to give reagents, which are then mixed with a sample to give the reaction complex. Each of the materials is brought into spatial proximity by binding based on the affinity among the materials having weak affinity for each other in the reaction complex, and since that condition is stably maintained, a more efficient energy transfer occurs.

Analysis method using reporter (label) intermolecular interaction

In an inexpensive and convenient method for immobilizing a suspension cell, a phospholipid vesicle or the like regardless of the type of cell, on the surface of a solid phase, a cell is immobilized by causing the cell to contact a support having a hydrophobic chain and a hydrophilic chain.

Immobilized cells and liposomes and method of immobilizing the same

This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptide onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.

Method for measuring antigen concentration

This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptides onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.

A method of feeding substrates into a tubular bioreactor incorporating a reacting vessel and a separating membrane in which the reaction and separation or collection can be continuously and surely effected in the same vessel with high efficiency.

Method of feeding a substrate into tubular bioreactor

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Saitama, Japan

Teruyuki Nagamune