Tokyo Women's Medical University

Nihon Kohden Corporation

Other

Dai Nippon Printing Co., Ltd.

Asahi Kasei Medical Co., Ltd.

Cellseed, Inc.

University of Utah Research Foundation

Asahi Kasei Kabushiki Kaisha

Kyoto University

Research Development Corporation of Japan

Terumo Corporation

The General Corporation

Able Corporation

The Jikei University

Kanoncure, Inc.

Teruo Okano

Nobuyuki Tanaka

Katsuyoshi Suzuki

Akira Masuda

Tatsuya Shimizu

Masayuki Yamato

Hirotsugu Kubo

Mitsuhiro Oura

Yoshikatsu Akiyama

Sunao Takeda

Yasuhisa Sakurai

Masanao Watanabe

Akane Suzuki

Akihiko Kikuchi

Ichiro Koguma

Hideko Kanazawa

Katsuhisa Matsuura

Hidekazu Sekine

Hideki Mukuno

Hiroki Shigematsu

Hidekazu Imai

Shoichirou Matsuhisa

Fumihiro Yamashita

Kiyoshi Kanai

Kazumi Tashiro

Yoshikazu Matsushima

Hideaki Arita

Kazuo Okuyama

Kenichi Nagase

Masanori Wada

Masayuki Yokoyama

Shinji Yamamori

Sung Wan Kim

Kazunori Kataoka

Kohji Nishida

Takahiro Shioyama

Yuji Haraguchi

You Han Bae

Noriko Yamada

Shohei Inoue

Mitsuo Umezu

Kazuo Ohashi

Masatoshi Kuroda

Jun Yamashita

Yukito Yamasaki

Isao Shinohara

Goshi Shiota

Hiroyuki Saimoto

Katsuhide Tone

Hironobu Takahashi

Minoru Morimoto

Hiroya Watanabe

Masahisa Ikemi

Katsuhisa Sakaguchi

Isao Ishikawa

Takao Hanawa

Yusuke Tsutsumi

Hiromi Kojima

Kenichi Hagiwara

Hiroshi Moriyama

Makoto Kaneko

Nobuo Kanai

Hidetoshi Masumoto

Yoshiko Hoshikawa

Yuka Tsumanuma

Noriko Matsumoto

Yoshiaki Matsumi

Ushio Iwamoto

Supreda Suphanantachat

Takayuki Tonoi

Kosei Yano

Masamichi Nakayama

Michi Sato

Mika Utsumi

Ai Kushida

Chie Konno

Kanako Konishi

Keisuke Ashiba

Masatoshi Oka

Takanori Hama

Sachiko Sekiya

Goro Kuramoto

Soichi Takagi

Kosaku Nitta

Shimizu Tatsuya

Ryohei Uchida

Makoto Kondo

Kaoru Washio

Hiroyuki Tsurii

Yamato Masayuki

Ashiba Keisuke

Watanbe Hiroya

Akiyama Yoshikatsu

A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.

Cell culture system and cell culture method

The present invention provides a method of inhibiting progression of or preventing renal disorder, the method comprising applying a cell sheet composition for inhibiting progression of or preventing renal disorder to at least one part of the surface of a kidney, wherein the part of the surface is uncovered by a fibrous capsule of the kidney. The present invention also provides a cell sheet composition for inhibiting progression of or preventing renal disorder, the cell sheet composition comprising a cell that has a function of producing a hepatocyte growth factor (HGF). The present invention also provides a method of producing a cell sheet composition for inhibiting progression of or preventing renal disorder.

Cell sheet composition for inhibiting progression of renal disorder, method of producing the same, and method of inhibiting progression of renal disorder using the same

A complex of implant and cultured periodontal ligament cell sheet, that can be satisfactorily stabilized into the bone through periodontal ligament tissue, is provided. A fixture of the inserted implant is coated with calcium phosphate and the cultured periodontal ligament cell sheet is brought into intimate contact with the implant.

Complex of implant and cultured periodontal ligament cell sheet, method for manufacturing the same, and method for using the same

The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80&#xb0; C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.

Middle ear mucosa-like cell sheet, process of producing the same and method of using the same

An object of the present invention is to provide a cell composition for treatment of uterine tissue that heals damage occurring in uterine tissue, including intrauterine adhesion, to a state that enables pregnancy. In addition, an object of the present invention is to provide a method for producing this cell composition for treatment of uterine tissue, and a cell composition for treatment of uterine tissue produced according to that method. The cell composition, which has a first cell layer containing epithelial cells and a second cell layer containing stromal cells, and the first cell layer is laminated on the second cell layer, has the ability to heal damage occurring in uterine tissue, including intrauterine adhesion, and restore it to a state that enables pregnancy.

Cell composition for treatment of uterine tissue and method for producing same

Provided is a means for evaluating the wetting characteristic of an object such as a cell sheet and a culture dish in a non-contact fashion. The wetting characteristic of an object is evaluated by a method comprising the steps of: (1) removing a liquid by jetting a gas at a surface of the object covered with the liquid, (2) measuring a dimension of a region in which the liquid is removed after the completion of the gas jetting and (3) evaluating the wetting characteristic of the object using the measured dimension as an index.

Method of evaluating wetting characteristic of object

Provided are layered cell sheets, comprising a plurality of layered cell sheets containing myoblasts, in which each cell sheet comprises cell population containing myoblasts with controlled orientations. Preferably provided are the layered cell sheets comprising a region in which the orientations of the cell population containing the myoblasts in each cell sheet are identical to each other.

Layered cell sheets containing myoblasts and method for producing same

With the present invention the sheet shaped therapeutic substance can be accurately and easily affixed to the inside of the human body. As shown in FIG.A, the sheet shaped substance can be mounted on the head with wrinkles being formed. When this takes place, the sheet shaped substance is held in contact with the outer periphery of the head. Then, by making the pressure of the fluid inside the vent apertures positive, the sheet shaped substance can be blown off. The surface of the head is closely parallel to an inner surface of the esophagus wall for the sheet shaped substance to be affixed. Therefore, this particularly makes it possible to perform such applying step (FIG.B) in a more reliable fashion.

Sheet shaped therapeutic substance transfer apparatus and method of affixing sheet shaped therapeutic substance

A cell culture apparatus includes: an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed; a sampling unit configured to sample the culture solution in the cell incubator; a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.

Cell culture apparatus

A measuring unit which is to be attached to a cell culture container configured to accommodate cells to be cultured and a culture solution, includes: a measurer which is configured to measure information related to the cells and the culture solution, in a non-contact manner; and a sensor which is configured to detect at least one of a position, a posture, an impact, an orientation, and vibration.

Measuring unit to be attached to cell culture container, cell culture container, and cultivation condition monitoring system

Provided is a temperature responsive adsorbent prepared by immobilizing a copolymer containing at least N-isopropylacrylamide to a base material surface. The copolymer has at least a strong cation exchange group. In addition, the copolymer contains the strong cation exchange group in an amount of 0.01 to 5 mol % relative to N-isopropylacrylamide in terms of monomer.

Temperature responsive adsorbent having a strong cation exchange group and method for producing the same

The present invention provides a method for producing a myocardial sheet using a group of cells derived from embryonic stem cells. This method is characterized by mixing Flk/KDR positive cells, cardiomyocytes, endothelial cells, and mural cells, all derived from embryonic stem cells, and culturing the mixed cells. Furthermore, the myocardial sheet can be used as a therapeutic agent for heart diseases since VEGF is released from the sheet.

Method for producing myocardial sheet from embryonic stem cell

A method for manufacturing a multilayered cell sheet characterized in fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues.

Method for manufacturing multilayered cell sheet, multilayered cell sheet having vascular network obtained thereby, and method of use thereof

Disclosed herein is a cell culture apparatus that can achieve appropriate culture conditions. The cell culture apparatus () includes: a cylindrical culture vessel () that holds a culture liquid containing cells; a supporting column () that stands upright in a center of an inner surface of a bottom () in the culture vessel; and a stirring device () that includes an attaching portion () that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade () whose upper portion is fixed to the attaching portion so as to rotate around the supporting column.

Cell culture apparatus and cell culture method using the same

Growing community of inventors

Tokyo, Japan

Teruo Okano