Mitsui Chemicals, Inc.

Teruo Arii

Takeshi Fukuda

Kiyoshi Ito

Toshifumi Yamaki

Takeshi Nakamura

Miyuki Tsuruoka

Toshihiro Oikawa

Takeya Abe

Masami Osabe

Katsuyuki Takahashi

Hiroko Shibamoto

Toshikazu Aikawa

Masanori Muramoto

Souichi Hazama

A method for producing an amide compound from a nitrile compound in an aqueous medium in the presence of a catalyst having a nitrile hydratase activity wherein the concentration of benzene in the aqueous medium is 4.0 ppm or less and a method for producing an amide-based polymer excellent in quality from the amide compound. Also provided are a method for more efficiently producing an acrylamide with higher quality by a microbial catalyst containing a nitrile hydratase and the like and a method for producing an acrylamide-based polymer, which is excellent in hue, has a good balance between water solubility and high molecular weight and is excellent in quality.

Method for producing amide compound

This invention provides a novel D-aminoacylase obtained by cloning a DNA encoding the novel D-aminoacylase from Methylobacterium mesophilicum MT 10894, etc. and which shows sufficiently high activity at an industrially useful substrate concentration to allow a D-amino acid to be efficiently produced from an N-acyl-DL-amino acid; a DNA encoding the D-aminoacylase; a process for producing a D-amino acid from the corresponding N-acylamino acid using a transformant containing the DNA.

DNA encoding novel D-aminoacylase and process for producing D-amino acid by using the same

The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.

Nitrile hydratase

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Chiba, Japan

Teruo Arii